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pBAD-pHScarlet

Plasmid

#191258

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet

Depositor

Robert Campbell

Insert

pHScarlet

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2 SH2-SH3

Plasmid

#46442

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 (GRB2 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 1-217

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pETDuet-T7-Ct-YhjH

Plasmid

#79167

Purpose

Dual expression of Ct biosensor and YhjH under individual T7 promoters

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Inserts

yhjH
T7-Spinach2-Ct

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp3 C163A-His

Plasmid

#11822

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C163A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
[pSK131] pCOLADuet p14|MP1

Plasmid

#136145

Purpose

bacterial expression of p14 and MP1

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

p14 and MP1

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pALACE

Plasmid

#31853

Depositor

Yossef Av-Gay

Article

Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

Type

Empty backbone

Use

Escherichia coli-mycobacterium shuttle plasmid

Tags

6xHIS

Expression

Bacterial

Promoter

Acetamidase operone

Available Since

July 9, 2012

Availability

Academic Institutions and Nonprofits only
pREDusk-AmpR-MCS

Plasmid

#188974

Purpose

For red light-repressed bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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HA-mDyn1 pcDNA3

Plasmid

#36263

Depositor

Pietro De Camilli

Article

Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

Insert

Dynamin 1 (Dnm1 Mouse)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 20, 2012

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaMIgG-14

Plasmid

#228994

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-14

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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