30,410 results

1. pNuc-cis

   Plasmid

   #202796

   Purpose

   RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

   Depositor

   David Edgell

   Article

   Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

   Insert

   TevSpCas9, sgRNA cassette, oriT, CenArsHis
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVSV eGFP dG

   Plasmid

   #31842

   Depositor

   Connie Cepko

   Article

   Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   VSV genome
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEvol-pAzFRS.2.t1

   Plasmid

   #73546

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAzFRS.2.t1
   pAzFRS.2.t1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBeloBac11loxP2272

   Plasmid

   #60342

   Purpose

   This is a BAC vector that contains both wt and mutant loxP sites.

   Depositor

   Jon Coren

   Article

   Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pComb3XTT

   Plasmid

   #63891

   Purpose

   Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Insert

   TT Fab
   

   Use

   Phage display

   Tags

   6xHis, HA tag, and geneIII

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTTCC

   Plasmid

   #164264

   Purpose

   Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

   Depositor

   Gudmundur Hreggvidsson

   Article

   Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

   Inserts

   Cas9
   gRNA scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pcel and trc

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDG02583

   Plasmid

   #104129

   Purpose

   Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   NEDD8-specific protease 1 (NEDP1)
   

   Use

   Tags

   14x Histidine tag, Maltose binding protein, and S…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC28-DENV3-NS5FL

   Plasmid

   #86013

   Purpose

   Expression of dengue virus 3 NS5 protein

   Depositor

   Dahai Luo

   Article

   Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

   Insert

   DENV3-NS5FL
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   amino acid residues 6 to 895 of DENV3 NS5

   Promoter

   T7

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCL149

   Plasmid

   #134457

   Purpose

   CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.

   Depositor

   Thomas Bernhardt

   Article

   Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

   Insert

   Para-popZ-rbs-H3H4-msfGFPN-CTM.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Para

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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