30,235 results

1. p307HU

   Plasmid

   #27386

   Depositor

   David Bartel

   Article

   Shechner et al Science. 2009 Nov 27. 326(5957):1271-5.

   Insert

   unligated form of ligase ribozyme
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-EC12WTC9AAvi

   Plasmid

   #47925

   Purpose

   Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

   Depositor

   Tanja Kortemme

   Article

   Ritterson et al J Am Chem Soc. 2013 Aug 7.

   Insert

   E-Cadherin EC12
   

   Use

   Tags

   6xHis, Avi Tag, and TEV Cleavage

   Expression

   Bacterial

   Mutation

   changed Cysteine 9 to Alanine

   Promoter

   T7

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbB5a-GFP

   Plasmid

   #35352

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR4-EGFP_opt

   Plasmid

   #106334

   Purpose

   Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   codon optimized for gene synthesis without affect…

   Promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Reb_81 aka PAS622

   Plasmid

   #71227

   Purpose

   Reb_1 with RebB(T28A, D72G)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebB(T28A, D72G), RebA(G53A)

   Promoter

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAJT248

   Plasmid

   #202289

   Purpose

   RPU integration plasmid (LPattB2)

   Depositor

   Christopher Voigt

   Article

   Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

   Insert

   J23101-RiboJ00-B0034-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSEVA12TEC-I

   Plasmid

   #58308

   Purpose

   GeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Insert

   Genomic cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

   Plasmid

   #204794

   Purpose

   Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   West Nile NS2B-NS3 fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

   Plasmid

   #198599

   Purpose

   Green fluorescent Ca2+ ion indicator for bacterial expression.

   Depositor

   Robert Campbell

   Article

   Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

   Insert

   HaloGFP-143-144
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK564

    Plasmid

    #110613

    Purpose

    BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Nanoluc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. gNS2B47NS3 S135A

    Plasmid

    #155317

    Purpose

    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutant

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    S135A

    Promoter

    M13

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ki67∆repeats-mNeonGreen-IRESpuro2

    Plasmid

    #183742

    Purpose

    Expresses Ki67∆repeats-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67(lacking repeats) (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67(lacking repeats)

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB3C5-phzSM

    Plasmid

    #141105

    Purpose

    Contains a phzS-phzM operon BioBrick

    Depositor

    Martin Buck

    Article

    Clifford et al Chem Sci. 2021 Jan 15;12(9):3328-3338. d

    Insert

    phzSM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTn7xKS

    Plasmid

    #117393

    Purpose

    Tn7 tagging vector with a LacI-controlled kill switch counterselection system

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. csr-1_son-1_mTagBFP2

    Plasmid

    #191746

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Use

    Tags

    NCU04502 promoter and mTagBFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MCV

    Plasmid

    #81174

    Purpose

    MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-EPN-11

    Plasmid

    #79926

    Purpose

    Palm1-Late1-I3-01-myc

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-11
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pET26-Ufd1-His

    Plasmid

    #21266

    Depositor

    Hemmo Meyer

    Article

    Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

    Insert

    Ufd1 (Ufd1l Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQLinkHD_mVenus

    Plasmid

    #118861

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    mVenus
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NEK2

    Plasmid

    #39163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NEK2 (NEK2 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    T175A inactivating mutant

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only