30,875 results

1. pGGC_RT_mScarlet

   Plasmid

   #136967

   Purpose

   Goldengate/Greengate CDS module, containing mScarlet

   Depositor

   Klaus Theres

   Insert

   mScarlet
   

   Expression

   Bacterial

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD18-Gm

   Plasmid

   #65100

   Purpose

   bacterial expression vector with Gentamycin selection, based on pBAD18

   Depositor

   Juan Tomas

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pJUMP46-2A(sfGFP)

   Plasmid

   #126990

   Purpose

   Level 2 vector. sfGFP reporter. Origin p15A (medium copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-PS-Grb2 1-154 (XSB382)

   Plasmid

   #78510

   Purpose

   Construct for expressing Grb2 lacking the C-terminal SH3 domain in E. Coli

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   Grb2 1-154 (GRB2 Human)
   

   Tags

   GST followed by a prescission protease claevage s…

   Expression

   Bacterial

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. PARP1-c009

   Plasmid

   #216872

   Purpose

   Bacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

   Depositor

   Lari Lehtiö

   Article

   Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

   Insert

   PARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
   

   Expression

   Bacterial

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB2-mRuby3

   Plasmid

   #104004

   Purpose

   Plasmid encoding mRuby3

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mRuby3
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. H14_MBP_SUMO-Cys-linker-BAF

   Plasmid

   #101777

   Purpose

   Expresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Tags

   His14-MBP-SUMO

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAWP78-PVCpnfΔpvc15_pvc12-HiBiT

   Plasmid

   #198309

   Purpose

   Negative control for payload loading assay; produces PVCs deficient for payload loader (pvc15)

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnfΔpvc15_pvc12-HiBiT
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK1E-RNAP

   Plasmid

   #48144

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase K1E
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET StrepII TEV LIC cloning vector (2R-T)

    Plasmid

    #29717

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    StrepII-TEV

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. PIM2

    Plasmid

    #38867

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PIM2 (PIM2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGEX_hDDX5_FL_D248N

    Plasmid

    #88872

    Purpose

    inducible expression of GST-DDX5 D248N in bacteria

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D248N

    Promoter

    Tac

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRAV23

    Plasmid

    #67715

    Purpose

    Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification

    Depositor

    Robert Batey, Jeffrey Kieft

    Article

    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

    Insert

    Transcription cassette for 3'-tagged RNAs
    

    Expression

    Bacterial

    Promoter

    T7 RNA polymerase

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pHLM

    Plasmid

    #64168

    Purpose

    harbours marker genes HIS3, LEU2 and MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sender plasmid

    Plasmid

    #127677

    Purpose

    Encodes the synthase LuxI for the quorum-sensing molecule C6-HSL.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

    Insert

    LuxI
    

    Use

    Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Promoter

    pT7

    Available Since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PTGDSA

    Plasmid

    #42452

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTGDSA (PTGDS Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. PET-3a-chpBK

    Plasmid

    #155201

    Purpose

    To express recombinant ChpBK protein

    Depositor

    Guanzheng Luo

    Article

    Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

    Inserts

    chpS-chpBK-6×His-Tag
    GyrA-CBD-10xHis-Tag
    

    Tags

    10xHis and 6xHis

    Expression

    Bacterial

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSAN-QUE20C

    Plasmid

    #129316

    Purpose

    QUEEN ATP sensor for 20degC

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-20C
    

    Expression

    Bacterial

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. KG#121

    Plasmid

    #110879

    Purpose

    Expresses the C. elegans unc-31 cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

    Inserts

    rab-3 promoter
    unc-31 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Mutation

    Missing first 408bp

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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