30,235 results

1. pNCS Citrine

   Plasmid

   #91761

   Purpose

   Bacterial expression of a fluorescent protein, Citrine

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Insert

   Citrine
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSY6

   Plasmid

   #90482

   Purpose

   Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum

   Depositor

   Sheng Yang

   Article

   Shao et al Cell Res. 2007 Nov;17(11):963-5. doi: 10

   Insert

   group II intron and ItrA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQTEV-RAB17

   Plasmid

   #34812

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   RAB17 (RAB17 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbB11a-GFP

   Plasmid

   #126114

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23150

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b-mEB2-eGFP-6xHis

   Plasmid

   #130982

   Purpose

   Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB2-GFP-6xHis (Mapre2 Mouse)
   

   Use

   Tags

   eGFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PhdeA-cfp

   Plasmid

   #127940

   Purpose

   CFP fluorescent reporter for HdeA

   Depositor

   Mary Dunlop

   Article

   Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

   Insert

   PhdeA-cfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP2822

   Plasmid

   #160670

   Purpose

   Mobile CRISPRi sfGFP 'test', empty sgRNA

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Insert

   empty sgRNA (BsaI)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Met1 diUb (1-152)

   Plasmid

   #110766

   Purpose

   Bacterial expression of Met1 diUb

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYC-PLlambda-alkS(WT)

   Plasmid

   #44382

   Depositor

   Hal Alper

   Article

   Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

   Insert

   AlkS(WT)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLlambda

   Available since

   July 15, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHAGE2-TetOminiCMV-Sox2

    Plasmid

    #136612

    Purpose

    Dox-inducible lentiviral vector expressing mouse Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET FLIPglu-600uDelta13

    Plasmid

    #13559

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A Delta13
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    F16A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbA8k-RFP

    Plasmid

    #35273

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-miniR-DBCO

    Plasmid

    #181984

    Purpose

    Used to label proteins with the MiniE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    MiniR-DBCO
    

    Use

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid

    #128404

    Purpose

    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A (toxA P. aeruginosa)
    

    Use

    Tags

    6xHis tag and DsbA signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-SUMO-SpyTag003

    Plasmid

    #216282

    Purpose

    Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SUMO-SpyTag003
    

    Use

    Tags

    His6 and SpyTag003

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNIC-HIS6-THRX-TEV-GG

    Plasmid

    #215796

    Purpose

    Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pDONR201-Eps8L2

    Plasmid

    #196526

    Purpose

    Entry vector for Gateway with Eps8L2

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2-FL (EPS8L2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-2T-GST-RNMT

    Plasmid

    #112709

    Purpose

    Expresses N-terminally GST-tagged human RNMT in bacterial cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Use

    Tags

    GST and thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEB1-mClover3

    Plasmid

    #103994

    Purpose

    Plasmid encoding mClover3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mClover3
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22B_PcrA_2Bchimera

    Plasmid

    #102998

    Purpose

    Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

    Depositor

    Martin Webb

    Insert

    PcrA_2Bchimera
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Bacilllus stearothermophilus PcrA in which the 2B…

    Promoter

    T7

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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