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pNDGG051

Plasmid

#231320

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG053

Plasmid

#231322

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG011, pOGG012, and pOGG014, NmR/KmR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMBA328

Plasmid

#214748

Purpose

Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RBS(22821)-mcherry

Expression

Bacterial

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA333

Plasmid

#214745

Purpose

Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-ISG15 C78SN13Y

Plasmid

#165106

Purpose

bacterial expressio of a GST fusion of Human ISG15 C78SN13Y

Depositor

Arthur Haas

Article

Narasimhan et al J Biol Chem. 2005 Jul 22. 280(29):27356-

Insert

ISG15 (ISG15 Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

C78SN13Y; The sequence of ISG15-C78S/N13Y contai…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCON1-ProB.mCherry

Plasmid

#112520

Purpose

ProB driven mCherry expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

ProB mCherry TT

Expression

Bacterial

Promoter

ProB

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-p53 R175H

Plasmid

#39480

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

TP53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

P72R and R175H

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only
pAWP78-PVCpnf_pvc13-Ad5RGDPK7_Δpvc10

Plasmid

#198449

Purpose

Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions); also contains truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5RGDPK7_Δpvc10

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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SUMO_TwinStrep_Csx30

Plasmid

#197611

Purpose

For protein purification of Csx30

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Csx30

Expression

Bacterial

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express

Plasmid

#103995

Purpose

Plasmid encoding DsRed-Express

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMS30: pTarget(PmcCAST)

Plasmid

#168163

Purpose

Target plasmid containing PmcPSP1 and attachment site for PmcCAST.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PAM for PmcCAST + PmcPSP1
tRNA-Val

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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ASC-CASP1 Octamer

Plasmid

#164032

Purpose

Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CASP1, ASC (CASP1, PYCARD Human)

Tags

His6-MBP-TEV

Expression

Bacterial

Mutation

W169G (ASC), G20K (CASP1)

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SpSuc1

Plasmid

#162558

Purpose

Expresses GST-tagged S.pombe Suc1 in E.coli

Depositor

Iain Hagan

Article

Singh et al Methods Mol Biol. 2021;2329:123-142. doi

Insert

Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEND-193_Cacnes_HS

Plasmid

#225622

Purpose

C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

HspR

Use

Synthetic Biology

Tags

pFAST-6XHis

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-229_Cacnes_EforCP

Plasmid

#225615

Purpose

Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

EforCP

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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BPTI-[5,55]Gly14Ile38

Plasmid

#195059

Purpose

Thermal stability of BPTI

Depositor

Yutaka Kuroda

Article

Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi:

Insert

bovine pancreatic trypsin inhibitor

Tags

His tag and trpΔLE leader sequences

Expression

Bacterial

Mutation

Changed Alanine residues at position of 14 and 38…

Promoter

T7 promotor

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0835:Dendra

Plasmid

#174923

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0835

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

Plasmid

#180156

Purpose

tdTomato cre reporter with Hipp11 recombineering homology arms

Depositor

Mario Capecchi

Insert

tdTomato

Use

Cre/Lox and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET3a-Ub-K48C

Plasmid

#18897

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin-K48C (UBC Human)

Expression

Bacterial

Mutation

Lysine 48 is replaced with a Cysteine residue

Available Since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only