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pGBHPS-SortA

Plasmid

#21930

Depositor

Fuyuhiko Inagaki

Article

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase

Tags

GB1, HRV3C cleagage site, and His

Expression

Bacterial

Mutation

deleted amino acids 1-59

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCis_2+7+8+5

Plasmid

#60589

Purpose

a 4-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+7+8+5

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTEV5_ RpMatB_2

Plasmid

#78206

Purpose

Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding

Depositor

Martin Webb

Article

Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

Insert

matB (His6 C106A S170A R286C Q457C K488A)

Tags

His6

Expression

Bacterial

Mutation

C106A S170A R286C Q457C K488A

Promoter

T7

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIKpBAD

Plasmid

#129273

Purpose

Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoter

Depositor

Lindsey Burbank

Article

Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEM112B

Plasmid

#210602

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8

Tags

mClover3

Expression

Bacterial and Yeast

Promoter

SpH2A/Bpro (divergent)

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRAV23

Plasmid

#67715

Purpose

Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
pTpal-fdc

Plasmid

#78288

Purpose

Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

Depositor

David Nielsen

Article

McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

Inserts

PAL2 (PAL2 Mustard Weed)
FDC1 (FDC1 Budding Yeast)

Expression

Bacterial

Mutation

*see below

Promoter

trc

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR:D70E

Plasmid

#79164

Purpose

Expresses constitutively active WspR D70E under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Mutation

D70E

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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EPHA7 (2REI)

Plasmid

#25148

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

epha7.590.899 (EPHA7 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pKM586

Plasmid

#19978

Depositor

David Waugh

Article

Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

Insert

TEV protease (TEVgp1 tobacco etch virus)

Expression

Bacterial

Mutation

S219D mutant

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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pEB1-mCerulean3

Plasmid

#103973

Purpose

Plasmid encoding mCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTHSSe_52

Plasmid

#109250

Purpose

ColE1 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-mdm2/2702

Plasmid

#11492

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Stat5A-SH2

Plasmid

#46515

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5A (STAT5A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 582-693

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCMU-4

Plasmid

#172353

Purpose

CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

Depositor

Eriko Takano

Article

Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

Insert

D4-TheoR-cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

D4

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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MS2_SC2_S_Delta

Plasmid

#175950

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

Depositor

Paul Freemont

Inserts

Maturation Protein
Coat Protein Dimer
S gene (S SARS-CoV-2)

Expression

Bacterial

Mutation

Delta (Amino Acid 319-869)

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRA1EGFP

Plasmid

#193791

Purpose

Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-ald*-alaE

Plasmid

#134939

Purpose

Low phosphate inducible alanine dehydrogenase and alanine exporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

alanine dehydrogenase and L-alanine exporter

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pYTK089

Plasmid

#65196

Purpose

Encodes AmpR-ColE1 as a Type 8a part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

AmpR-ColE1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Tags

His

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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