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pTHSSe_51

Plasmid

#109249

Purpose

p15A BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS1c-RFP

Plasmid

#35338

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Trc

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pSB3C5-phzSM

Plasmid

#141105

Purpose

Contains a phzS-phzM operon BioBrick

Depositor

Martin Buck

Article

Clifford et al Chem Sci. 2021 Jan 15;12(9):3328-3338. d

Insert

phzSM

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFC331

Plasmid

#87843

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
argB

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and native pro…

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEN12A-P1

Plasmid

#27366

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

Pmyc1tetO

Use

Gateway donor vector

Expression

Bacterial

Mutation

N/A

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15

Plasmid

#153523

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#15

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#15

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA(D130N)-RagC

Plasmid

#99642

Purpose

Bacterial expression of RagA(D130N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

D130N

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Reb_81 aka PAS622

Plasmid

#71227

Purpose

Reb_1 with RebB(T28A, D72G)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebB(T28A, D72G), RebA(G53A)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pET28-MRPP1

Plasmid

#67862

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP1 (TRMT10C Human)

Tags

His

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pTK164

Plasmid

#13533

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTY

Tags

ECFP and Venus

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pEF1alpha-mClover3-bGHpA

Plasmid

#172286

Purpose

Constitutive expression of mClover3

Depositor

Patrik Verstreken

Insert

mClover3

Expression

Bacterial

Promoter

EF1alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC-CH10-GG

Plasmid

#215807

Purpose

Cloning of target gene with C terminal non cleavable His10 tag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pE3c

Plasmid

#17463

Purpose

modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Article

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

6xMyc

Expression

Bacterial, Insect, Mammalia…

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-mRFP1-kanMX6

Plasmid

#133551

Purpose

Yeast fluorescent (RFP) vector with PTEF promoter.

Depositor

Won-Ki Huh, Erin O'Shea

Article

Huh et al Nature. 2003 Oct 16;425(6959):686-91. do

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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RFP-(SIM)1

Plasmid

#127094

Purpose

Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

mCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Tags

mCherry

Expression

Bacterial

Promoter

unknown

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEBG-NHERF2

Plasmid

#28292

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF2 (NHERF2 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pFPV-mCherry-TT

Plasmid

#112523

Purpose

PrpsM driven mCherry expression with synTT

Depositor

Olivia Steele-Mortimer

Article

Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

Insert

PrpsM mCherry TT

Expression

Bacterial

Promoter

PrpsM

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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KG#203

Plasmid

#110880

Purpose

Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJF3H

Plasmid

#63897

Purpose

For phage display of cDNA libraries

Depositor

Carlos Barbas

Article

Barbas et al Cold Spring Harbor, NY: Cold Spring Harb

Insert

cDNA cloning region

Use

Phage display

Tags

Fos

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pT7C-HSPA1L

Plasmid

#177660

Purpose

Recombinant protein synthesis of human HSPA1L

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSPA1L (HSPA1L Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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