32,684 results
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Plasmid#171225PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)DepositorInserthuman androgen receptor Ser90Ala mutant (AR Human)
ExpressionBacterial and MammalianMutationAR-Ser90Ala (AR-S90A)PromoterCMVAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
TagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559Available SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3XP3.RFP-tan
Plasmid#83474PurposeRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsert3XP3.RFP-tan
UseCRISPRExpressionBacterial and InsectPromoter3XP3 eye-expression PromoterAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
HSPA1LA
Plasmid#42429PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK-GW
Plasmid#126929PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(D3A,V266E)
Plasmid#90091PurposeExpresses caspase D3A and V266E mutant in bacterial cellsDepositorAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b
Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRKO
Plasmid#49456PurposeloxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptionsDepositorInsertloxP-kanMX-loxP cassette
UseCre/LoxExpressionBacterialPromoternoneAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD18-Gm
Plasmid#65100Purposebacterial expression vector with Gentamycin selection, based on pBAD18DepositorTypeEmpty backboneExpressionBacterialPromoterpBADAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-UAP56
Plasmid#157661PurposeExpresses UAP56 in E.coli cellDepositorInsertSpliceosome RNA helicase DDX39B (DDX39B Human)
TagsGST tagExpressionBacterialMutationdeleted amino acids 1-43Available SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAK1.A
Plasmid#29460DepositorTypeEmpty backboneUseMycobacteria expressionExpressionBacterialPromoterHsp60Available SinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pC009 LshCas13a locus with targeting spacer
Plasmid#91900PurposeLshCas13a locus into pACYC184 with targeting spacerDepositorInsertLshCas13a locus with targeting spacer
ExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRBBm59
Plasmid#48115PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP BCHZ (1079-1751)
Plasmid#99341PurposeBacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)DepositorInsertmouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
TagsHis6tevExpressionBacterialPromoterT7Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
SND1 (SND1A-c003)
Plasmid#98242PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSND1A (SND1 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00567:TUDOR 677-799PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJDC124
Plasmid#157877PurposemCherry is expressed under the mycobacterial phsp60 heat shock promoter for in vivo and in vitro detection.DepositorInsertmCherry
ExpressionBacterialPromoterphsp60Available SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB2-Katushka
Plasmid#104008PurposePlasmid encoding KatushkaDepositorInsertKatushka
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (E3V, K6T, N21D, R3…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PERK 5: PERK.K618R.pGEX4T-1
Plasmid#21818DepositorInsertPERK (537-1114) K618R (Eif2ak3 Mouse)
TagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114. Kinase …Available SinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-OspC
Plasmid#159998PurposeExpresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cellsDepositorInsertSpyTag003-OspC (20-209) C129S
TagsHis6-tag and Thrombin cleavage siteExpressionBacterialMutationC129S mutation improves expression level.PromoterT7Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only