30,249 results

1. SS13 lac-inducible GFP reporter circuit

   Plasmid

   #30336

   Depositor

   Herbert Sauro

   Article

   Sleight et al J Biol Eng. 2010 Nov 1;4:12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaM-1

   Plasmid

   #172767

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-1
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRM2

   Plasmid

   #8486

   Depositor

   Nancy Craig

   Article

   McKown et al J Bacteriol 1988 Jan;170(1):352-8.

   Insert

   attTn7 (tnsA Bacteria)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMRE-Tn5-141

   Plasmid

   #118525

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX Stat6-SH2

   Plasmid

   #46517

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat6 (STAT6 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 510-654

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSAVED-CHAT_PCaspase_PCi_PC-σ

   Plasmid

   #214040

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-Pci-PC-σ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21(+)-His6-Ubc13

   Plasmid

   #212715

   Purpose

   Bacterial expression of His6-Ubc13

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Ubc13
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAllet

   Plasmid

   #202560

   Purpose

   E. coli cloning vector with large MCS (50+ unique restriction sites)

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH6HTC_CCDC59-Halo

   Plasmid

   #175327

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   CCDC59 (CCDC59 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. T5-sgRNA

    Plasmid

    #113655

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter T5 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. SMS

    Plasmid

    #25234

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SMS_01
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pBMTBX-1

    Plasmid

    #26072

    Depositor

    Ryan Gill

    Article

    Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBMT-4

    Plasmid

    #22837

    Depositor

    Ryan Gill

    Article

    Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX4T3-HA-ERK2 K54R

    Plasmid

    #53170

    Purpose

    Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Mustard Weed, Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    K54R

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pBEAST_J23101-bphC

    Plasmid

    #128137

    Purpose

    The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    bphC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. RGS16

    Plasmid

    #39140

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RGS16 (RGS16 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pTriEx-mCherry-Zdk1-RhoA Q63L

    Plasmid

    #81058

    Purpose

    mammalian expression of Zdk1 tagged RhoA Q63L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    RhoA Q63L (RHOA Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-7 asyn S129A

    Plasmid

    #36048

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein S129A (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S129A

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMal-E4rGel

    Plasmid

    #51280

    Purpose

    Expresses a fibronectin fusion with gelonin targeting EGFR in bacteria

    Depositor

    Dane Wittrup

    Article

    Pirie et al J Biol Chem. 2011 Feb 11;286(6):4165-72.

    Insert

    E4rGel
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbA4c-RFP

    Plasmid

    #35299

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProE

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge