30,880 results

1. pDH42(CUG/A)

   Plasmid

   #154132

   Purpose

   Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   engineered bacterial rRNAs operon under control of pL promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PTPN22

   Plasmid

   #38933

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PTPN22 (PTPN22 Human)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   G126V, V147G

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pUC19-link-mVenus-FLAG:Kan

   Plasmid

   #211830

   Purpose

   Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   mVenus-FLAG-Kan
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDM_noro_A2_lacZA

   Plasmid

   #118819

   Purpose

   T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch A2
   

   Expression

   Bacterial

   Available Since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GARS_pNIC-Bio3

   Plasmid

   #153049

   Purpose

   Expression construct for producing Avi tagged Aspartyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   GARS (GARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX SH2-B-SH2

   Plasmid

   #46479

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SH2-B (SH2B1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 515-639

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEasyG1_hph

   Plasmid

   #184912

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSP1D1R1

   Plasmid

   #97323

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1R1
   

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of R residue after T25

   Promoter

   T7

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCellFree_KA1_ZIKV NS2A

   Plasmid

   #145343

   Purpose

   Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term eGFP

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS2A
   

   Tags

   8xHis and eGFP

   Expression

   Bacterial

   Promoter

   T7- CMV/CAG

   Available Since

   Feb. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTXB1-hMeCP2

    Plasmid

    #48091

    Purpose

    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Expression

    Bacterial

    Mutation

    Mxe intein/chitin binding domain tagged

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pDEST17-UbE2U

    Plasmid

    #15810

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2U (UBE2U Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pHelper-CBE-sgRNA

    Plasmid

    #221137

    Purpose

    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with Cas9 handle
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119(SpeI)

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB-CBEv1

    Plasmid

    #221138

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Promoter

    lacIq-Ptac

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCOLA-Gent-yibDp-Erv1p

    Plasmid

    #202483

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM5082

    Plasmid

    #85121

    Purpose

    PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    PkaiB-KaiC-ECFP
    

    Tags

    ECFP

    Expression

    Bacterial

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMAL630

    Plasmid

    #167836

    Purpose

    Encodes OptoLAC3 circuit

    Depositor

    Jose Avalos

    Article

    Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

    Insert

    lacI
    

    Use

    Synthetic Biology

    Tags

    ssrA (AAV)

    Expression

    Bacterial

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PhdeA-cfp

    Plasmid

    #127940

    Purpose

    CFP fluorescent reporter for HdeA

    Depositor

    Mary Dunlop

    Article

    Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

    Insert

    PhdeA-cfp
    

    Expression

    Bacterial

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. STK24_HUMAN_D0

    Plasmid

    #79742

    Purpose

    This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK24 (STK24 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pBbA0c-RFP

    Plasmid

    #35373

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbS0k-RFP

    Plasmid

    #35369

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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