30,880 results

1. pFA6a-mMaple3-kanMX6

   Plasmid

   #105165

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

   Insert

   mMaple3-kanMX6
   

   Tags

   GlySerGly-mMaple3

   Expression

   Bacterial and Yeast

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGV3319

   Plasmid

   #170277

   Purpose

   Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

   Depositor

   Gregory Van Duyne

   Article

   Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

   Insert

   Bst-LF D720A
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   D720A

   Promoter

   T7-lac

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BB3rN_14*

   Plasmid

   #98550

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETx-SR6c3

   Plasmid

   #176211

   Purpose

   Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   SR6c3
   

   Tags

   6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET51b(+)_SNAP-p24

   Plasmid

   #130718

   Purpose

   Plasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid protein

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-p24
   

   Tags

   Hisx10 and StrepTag

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTriEx-mCherry::LANS1

   Plasmid

   #60784

   Purpose

   Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

   Insert

   LANS1
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET29b-Ava3858-3855

   Plasmid

   #45746

   Depositor

   Emily Balskus, Christopher T Walsh

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   Ava3858-3855
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pGEX4T2-Ste7-EE

   Plasmid

   #52681

   Purpose

   expression of constitutively active Ste7 kinase in bacteria

   Depositor

   Benjamin Turk

   Article

   Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

   Insert

   Ste7 (STE7 Budding Yeast)
   

   Tags

   GST and Myc

   Expression

   Bacterial

   Mutation

   S359E/T363E constitutively active

   Promoter

   tac

   Available Since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pECT3

   Plasmid

   #75450

   Purpose

   Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt3
   

   Expression

   Bacterial

   Promoter

   none

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4046910

    Plasmid

    #145672

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pETM33_MAD2L1_HORMA

    Plasmid

    #178480

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    MAD2L1_HORMA (MAD2L1 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTriEx-mVenus-Zdk1-RhoA Q63L

    Plasmid

    #81046

    Purpose

    mammalian expression of Zdk1 tagged RhoA Q63L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    RhoA Q63L (RHOA Human)
    

    Tags

    mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDH-CMV-MSI1(FL)

    Plasmid

    #60358

    Purpose

    express mouse MSI1 in mammalian cells

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (FL) (Msi1 Mouse)
    

    Expression

    Bacterial

    Promoter

    CMV

    Available Since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSF-1b-S14

    Plasmid

    #128596

    Purpose

    Expresses tagless 30S ribosomal subunit protein S14 in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S14 (rpsN E.coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTE104

    Plasmid

    #59399

    Purpose

    MethyloBrick expression vector, coxB promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PcoxB

    Available Since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR PKAKTRn-Clover

    Plasmid

    #177199

    Purpose

    Gateway entry vector encoding PKAKTRnew-Clover

    Depositor

    Markus Covert

    Article

    Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

    Insert

    PKAKTRnew-mClover
    

    Expression

    Bacterial

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAM5085

    Plasmid

    #85122

    Purpose

    PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
    

    Tags

    ECFP

    Expression

    Bacterial

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBW-m4046795

    Plasmid

    #145598

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pZH516

    Plasmid

    #102669

    Purpose

    Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Expression

    Bacterial

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTn7xKS-sfGFP

    Plasmid

    #117394

    Purpose

    Tn7 tagging vector pTn7xKS with sfGFP expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge