30,256 results

1. pEB1-mTurquoise2

   Plasmid

   #103974

   Purpose

   Plasmid encoding mTurquoise2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mTurquoise2
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F64L, Y66W, …

   Promoter

   proC

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T-1 3xHA-MKK4-EE

   Plasmid

   #47577

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK4-EE (MAP2K4 Human)
   

   Use

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Lacks the first 36 residues of the RefSeq protein…

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pBLxR5

   Plasmid

   #167504

   Purpose

   Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLuxB

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. YFP-FKBPx5-His

   Plasmid

   #103785

   Purpose

   expresses His-tagged iPOLYMER component in E. coli for protein purification

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   YFP-FKBPx5-His
   

   Use

   Tags

   EYFP, His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB021CH(+)

   Plasmid

   #99209

   Purpose

   Accessory plasmid for PACE of PylRS variants

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   PT7 [SD8] gIII, luxAB; ProK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PT7 and PproK

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR8/GW/TOPO PCGF1

   Plasmid

   #69770

   Purpose

   Gateway entry vector for PCGF1

   Depositor

   Gerard Cagney

   Insert

   PCGF1 (PCGF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-6P-1-DmMe31B_G

   Plasmid

   #146381

   Purpose

   Bacterial Expression of DmMe31B

   Depositor

   Elisa Izaurralde

   Insert

   DmMe31B (me31B Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBHSTC97

   Plasmid

   #124352

   Purpose

   Plasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin

   Depositor

   Hideo Iwai

   Article

   Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

   Insert

   NpuDnaBΔ283 mini-intein
   

   Use

   Tags

   CcdA-C (SFAD)

   Expression

   Bacterial

   Mutation

   Deletion of 283-residue endonuclease domain

   Promoter

   STC

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbS6c-RFP

   Plasmid

   #35293

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LlacO-1

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET51b-His-TEV-HaloTag7_dead_mt

    Plasmid

    #167267

    Purpose

    Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    D106A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PANK1

    Plasmid

    #25517

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PANK1 (PANK1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. TDRD3 (3PMT)

    Plasmid

    #28274

    Depositor

    Cheryl Arrowsmith

    Insert

    Tudor domain-containing protein 3 (TDRD3 Human)
    

    Use

    Tags

    His with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains Tudor domain, residues 553-611.

    Promoter

    Available since

    April 14, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pGEX2TK-Skm1-PH

    Plasmid

    #71371

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Skm1-PH
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa1-132

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

14. POLArIS_NbVim-cp10GFP

    Plasmid

    #171020

    Purpose

    Expresses NbVim-cp10GFP#2 in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

    Insert

    anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
    

    Use

    Tags

    cp10GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAV115

    Plasmid

    #63182

    Purpose

    pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MEF2A-Sensor

    Plasmid

    #208928

    Purpose

    in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    Mef2a docking sequence+Phospho site (MEF2A Human)
    

    Use

    Tags

    HISx6, MBP, and Smbit

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pES134

    Plasmid

    #127551

    Purpose

    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

    Depositor

    Emmanuele Severi, Gavin Thomas

    Article

    Wu et al Microbiology (Reading). 2024 Mar;170(3).

    Insert

    F3-flanked spectinomycin resistance cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dapp1-PH pET11a

    Plasmid

    #71376

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Dapp1-PH (DAPP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 148-273

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCAM12

    Plasmid

    #86471

    Purpose

    Expresses p12 and p9-mCitrine

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p12, p9-mCitrine
    

    Use

    Synthetic Biology

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2-Flag-TTGZFP-FokI-DD

    Plasmid

    #18755

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    zinc finger nuclease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RNA expression vector for expressing the zinc fin…

    Promoter

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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