30,256 results
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Plasmid#127196PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 Y24xF (FUS Human)
UseTagshexahistidineExpressionBacterialMutationY24xF: Y6F, Y14F, Y17F, Y25F, Y33F, Y38F, Y41F, Y…PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAmeR-F1
Plasmid#74675PurposeAmeR-F1 gateDepositorInsertPTac-RBSF1-AmeR-PAmeR-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-Vif
Plasmid#46313DepositorInsertsp19
Vif DNA-1
linker
Vif DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK314
Plasmid#110545PurposeModified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteriaDepositorInsertsModified PrhaBAD (CRP-binding sites removed)
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Nedd-4/2522
Plasmid#11466DepositorInsertNedd4-1 (Nedd4 Mouse)
UseTagsGSTExpressionBacterialMutationaa 52-777PromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBLO 4.01
Plasmid#121495Purposeengineered Mu transposonDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterp15AAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136464
Plasmid#149534PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15bLCFPT7
Plasmid#53545PurposeActivation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistantDepositorInsertT7 RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Omega 2
Plasmid#118047PurposeGoldenBraid2.0 Omega 2 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Alpha2
Plasmid#118045PurposeGoldenBraid2.0 Alpha 2 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Claspin-FL-wt
Plasmid#31621DepositorInsertClaspin (CLSPN Human)
UseTagsFlag, GST, and HisExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pHH03C_32
Plasmid#120897PurposeFunctional test of ECF sigma factors and their cognate promotersDepositorInsertECF32_1122
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mTurquoise
Plasmid#103972PurposePlasmid encoding mTurquoiseDepositorInsertmTurquoise
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH04_32_sRNA-A_Sp_coD
Plasmid#120900Purposethe component plasmid of a regulated deviceDepositorInsertspECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
sRNA-A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-mCherry
Plasmid#71737PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertmCherry
UseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterpRplsWTAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2NtB2
Plasmid#160881PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGCNK58
Plasmid#202461PurposePlasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cellsDepositorInsertResidues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
B1-2
Plasmid#111464PurposeMet-tRNA synthetaseDepositorInsertMet-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP
Plasmid#103997PurposePlasmid encoding TagRFPDepositorInsertTagRFP
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only