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pET28a-PSAT

Plasmid

#74115

Purpose

Expression of PSAT

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

pET28a-PSAT (PSAT1 Human)

Expression

Bacterial and Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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GST-FOXO1

Plasmid

#24379

Depositor

Anne Brunet

Insert

Foxo1 (Foxo1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

May 28, 2010

Availability

Academic Institutions and Nonprofits only

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pCAT202

Plasmid

#134879

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

deletion of Y208

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBYK01

Plasmid

#138656

Purpose

phiOZJ integrating Streptomyces expression vector lacking RDF

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTXG

Plasmid

#52280

Purpose

Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Type

Empty backbone

Tags

GyrA-intein-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pKM631

Plasmid

#17727

Depositor

David Waugh

Article

Kapust et al Biochem Biophys Res Commun. 2002 Jun 28.

Insert

NusG (nusG Aquifex aeolicus)

Tags

His6 and MBP

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pMVV108

Plasmid

#215338

Purpose

Expresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PSalTTC

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA334

Plasmid

#214746

Purpose

High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-ddFLN4-HIS-ybbr

Plasmid

#101239

Purpose

wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

Fgß peptide, ddFLN4 fingerprint (FGB D. discoideum, Human)

Tags

HIS and ybbr

Expression

Bacterial

Mutation

C18S in ddFLN4

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pIBB-mEGFP-IRES-puro2b

Plasmid

#26462

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pIBB

Tags

mEGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pTD-C_eYFPTwinStrep

Plasmid

#45942

Purpose

expresses E.coli codon optimized EYFP-Twin-Strep fusion protein

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Insert

synthetic eYFP (codon usage adapted to E.coli K12)

Tags

Twin-Strep-tag

Expression

Bacterial

Promoter

lacIq/Ptrc

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pL0-BvDODAα1

Plasmid

#162528

Purpose

Beta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

DODAα1

Use

Puc19-derived

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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SNTB2

Plasmid

#38912

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

SNTB2 (SNTB2 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-EGFAB

Plasmid

#25770

Depositor

Johann Deisenhofer

Article

Kwon et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

Insert

LDL receptor EGFAB (LDLR Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pGGG-Sr43

Plasmid

#186974

Purpose

Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

Depositor

Brande Wulff

Article

Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

Insert

Sr43

Tags

None

Expression

Bacterial and Plant

Mutation

None

Promoter

Natice

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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MBP-His6-TEV-MMS2

Plasmid

#25465

Depositor

Cynthia Wolberger

Article

Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

Insert

MMS2 (MMS2, UBC13 Budding Yeast)

Tags

His6 and MBP

Expression

Bacterial

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pACYC-PLlambda-alkS(WT)

Plasmid

#44382

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Insert

AlkS(WT)

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlambda

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pET21c(+)-mtPheRS

Plasmid

#31080

Depositor

Linda Spremulli

Article

Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

Insert

Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Nt …

Promoter

T7

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pL0M-S-tdTomato-EC18158

Plasmid

#137078

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

tdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Article

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Expression

Bacterial and Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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