30,880 results

1. pQE80L TriCatcher-ELP

   Plasmid

   #112630

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-ELP
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-StrepII-TagRFP-CohI

   Plasmid

   #58710

   Purpose

   Detection construct to label dockerin type I containing fusion proteins

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   TagRFP-CohI
   

   Tags

   StrepII

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-MAGE-2-429

   Plasmid

   #89129

   Purpose

   bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

   Depositor

   Elizabeth Wilson

   Article

   Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

   Insert

   MAGE-A11 (MAGEA11 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

4. RAB14

   Plasmid

   #25519

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RAB14 (RAB14 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pJMD13

   Plasmid

   #138653

   Purpose

   phiC31 integrating Streptomyces expression vector

   Depositor

   Joshua Blodgett

   Article

   Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   PermE* variant sequence from pDA1652

   Available Since

   June 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWA PAS-E BAD K-GAFm

   Plasmid

   #39866

   Depositor

   Vladislav Verkhusha

   Article

   Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

   Inserts

   iRFP protein PAS domain
   iRFP protein GAF domain (mutated)
   

   Tags

   E coil and K coil

   Expression

   Bacterial

   Mutation

   F165Y, D232Y, W308R

   Promoter

   arabinose inducible and rhamnose inducible

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-DEST-Myc

   Plasmid

   #119752

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Tags

   GST and Myc

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB078d9

   Plasmid

   #79207

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1+2+3); Ppro1

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD/HisD-TagRFP675

   Plasmid

   #44274

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

   Insert

   TagRFP675
   

   Expression

   Bacterial

   Mutation

   M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

   Promoter

   pBAD

   Available Since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT122

    Plasmid

    #31396

    Depositor

    Christopher Voigt

    Article

    Tabor et al J Mol Biol. 2011 Jan 14. 405(2):315-24.

    Insert

    pJT122
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pKB226

    Plasmid

    #170016

    Purpose

    Encodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Defensin HNP-1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS

    Plasmid

    #154229

    Purpose

    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)

    Depositor

    Arnold Driessen

    Article

    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

    Insert

    p40S:QfDBD-VP16AD-GFP-NLS
    

    Use

    Synthetic Biology; Fungal expression

    Tags

    GFP-NLS; DsRed-SKL

    Expression

    Bacterial

    Promoter

    p40S (AN0465 A. nidulans)

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. S5_EcoRV_Ef1a_AscI

    Plasmid

    #128469

    Purpose

    Ef1a promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    Ef1a promoter
    

    Expression

    Bacterial

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. APneg-SNAP25c

    Plasmid

    #168013

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Expression

    Bacterial

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. APneg-SNAP25b

    Plasmid

    #168012

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Expression

    Bacterial

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS chick islet1 [TJ#44]

    Plasmid

    #16273

    Depositor

    Thomas Jessell

    Insert

    islet1 (ISL1 Chicken)
    

    Expression

    Bacterial

    Available Since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pJUMP26-1A(sfGFP)

    Plasmid

    #126973

    Purpose

    Level 1 vector. sfGFP reporter. Origin p15A (medium copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRAU171

    Plasmid

    #86835

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA2

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA2
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJPC12-ΔM13-mCherry-P/PM,5C6A-GFP

    Plasmid

    #80910

    Purpose

    Reporter plasmid (mCherry and GFP)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    mCherry
    GFP
    

    Expression

    Bacterial

    Promoter

    P5C6A and PM,5C6A

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103 NEDD4L WW domain #2

    Plasmid

    #104205

    Purpose

    Bacterial expression of WW domain #2 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #2 (NEDD4L Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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