30,256 results

1. pAC-EsaR-D91G

   Plasmid

   #47646

   Purpose

   pAC-EsaR with a a272g mutation in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-D91G
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 91 to Glycine

   Promoter

   Plac

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pXY1-spc

   Plasmid

   #90488

   Purpose

   Plasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

   Depositor

   Sheng Yang

   Article

   Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HOXA7 (murine) His-tag pET

   Plasmid

   #8491

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HOXA7 (Hoxa7 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   Fusion sequence is: ggatccATG; makes protein by …

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. Hp MTAN

   Plasmid

   #69802

   Purpose

   Expression of H. pylori J99 methylthioadenosine nucleosidase

   Depositor

   Vern Schramm

   Article

   Wang et al Biochemistry. 2012 Sep 4;51(35):6892-4.

   Insert

   Methylthioadenosine nucleosidase
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLBH546_Tet-Cas14b1Locus

   Plasmid

   #112503

   Purpose

   Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T1 human TBC1D7 (19-293)

   Plasmid

   #132617

   Purpose

   expresses human TBC1D7 (19-293) in E. coli

   Depositor

   Wenqing Xu

   Article

   Qin et al J Biol Chem. 2016 Apr 15;291(16):8591-60

   Insert

   TBC1D7 (19-293)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC1750

   Plasmid

   #127984

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET41a-MED19

   Plasmid

   #15430

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED19 (Med19 Mouse)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pLN

   Plasmid

   #40028

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   loxP
   Neo
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   None and SV40

   Available since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZP17 Krox20

    Plasmid

    #16924

    Depositor

    Randall Moon

    Insert

    Krox20 (egr2b Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pYB-Dual(mNG(yeast-opt))

    Plasmid

    #216727

    Purpose

    Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

    Depositor

    Emil Jensen

    Article

    Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pGAL1(yeast) and pT7-RNAP(bacteria)

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))

    Plasmid

    #164262

    Purpose

    Vector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Insert

    Codon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

    Plasmid

    #164263

    Purpose

    Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpal2At

    Plasmid

    #78286

    Purpose

    Expresses A. thaliana PAL2 in E. coli

    Depositor

    David Nielsen

    Article

    McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

    Insert

    PAL2 (PAL2 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    *see below

    Promoter

    lac

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHis-zfCSF3b

    Plasmid

    #65613

    Purpose

    express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

    Insert

    Csf3b (csf3b Zebrafish)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pOPS0314

    Plasmid

    #115507

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. RG-001

    Plasmid

    #133439

    Purpose

    Heat 3 domain of human elF4G in pET21 backbone.

    Depositor

    Gerhard Wagner

    Insert

    EIF4G1 (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCellFree_KA2_ZIKV E

    Plasmid

    #145361

    Purpose

    Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Envelope
    

    Use

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SARS-CoV-2_N_deltaNTD

    Plasmid

    #157869

    Purpose

    express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

    Depositor

    Nicolas Fawzi

    Article

    Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

    Insert

    N (N SARS-CoV-2)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    deltaNTD

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC012 LwCas13a locus with nontargeting spacer

    Plasmid

    #91904

    Purpose

    LwCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with non-targeting spacer
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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