30,880 results

1. pCD227

   Plasmid

   #113318

   Purpose

   AraC-pBAD-dCas9, MCP-SoxS_R93A

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   dCas9 and MCP-SoxS_R93A
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   R93A

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPath-pR-integrase

   Plasmid

   #200000

   Purpose

   Entry vector for biosynthetic pathway. pR-phiC31 integrase

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Pathways
   

   Expression

   Bacterial

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAmtR-A1

   Plasmid

   #74676

   Purpose

   AmtR-A1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSA1-AmtR-PAmtR-YFP
   

   Expression

   Bacterial

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046809

   Plasmid

   #145685

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAE03

   Plasmid

   #61044

   Purpose

   To construct C-terminal GFP fusions, single crossover integration vector

   Depositor

   Jan-Willem Veening

   Article

   Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   GFP+

   Expression

   Bacterial

   Available Since

   Feb. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. XRCC1-c003

   Plasmid

   #173090

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   XRCC1 (XRCC1 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E315-P406

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Fyn-SH3_5WT_S25C

   Plasmid

   #118824

   Purpose

   Bacterial expression of the SH3 domain of human Fyn

   Depositor

   Nicolas Fawzi

   Article

   Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

   Insert

   FYN (FYN Human)
   

   Tags

   6-His-TEV tag

   Expression

   Bacterial

   Mutation

   S25C; See depositor comments below.

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. His-PHD2-181-426

   Plasmid

   #223551

   Purpose

   His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-180.

   Promoter

   trc

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_PA4114_Cterm_His

   Plasmid

   #220484

   Purpose

   heterologous expression of PA4114 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   PA4114
   

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRha-tns

    Plasmid

    #170630

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTnsABC
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC301

    Plasmid

    #31851

    Depositor

    Yossef Av-Gay

    Article

    Cowley et al Gene. 2001 Feb 21;264(2):225-31.

    Insert

    GFP (including ATG start codon)
    

    Expression

    Bacterial

    Promoter

    SOD

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pUC-UAS1B4-Leum

    Plasmid

    #44312

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B4-Leum promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B4-Leum

    Available Since

    July 15, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. hsp70:mKate2-NWASP-CA

    Plasmid

    #105967

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    NWASP-CA
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBJG1 T7-8xHis-OGT D554N

    Plasmid

    #154279

    Purpose

    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    Aspartate 554 changed to Asparagine- bacterial co…

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. RGS16

    Plasmid

    #39140

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RGS16 (RGS16 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. 6974 pPROEX-HTb-SSB1

    Plasmid

    #1232

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    ssb1 (SSB1 Budding Yeast)
    

    Tags

    his tag (tev cleavable)

    Expression

    Bacterial

    Available Since

    Aug. 9, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pJK_proB_eGFP

    Plasmid

    #59845

    Purpose

    Constitutively expresses eGFP with proB promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    proB

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEE065

    Plasmid

    #176822

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    704
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJUMP19-Pac

    Plasmid

    #126957

    Purpose

    Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBSMrnaStrp

    Plasmid

    #67508

    Purpose

    Express the tRNAMet scaffold with the streptavidin aptamer tag

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNAMet with streptavidin aptamer
    

    Expression

    Bacterial

    Promoter

    lpp

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only