31,188 results
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Plasmid#132698Purposeexpresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISADepositorInsertnanobody Nb_TM#2
ExpressionBacterialPromoterpBADAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP6f-RS06
Plasmid#67159Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP6f-RS06
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationM374Q within GCaMP6fPromoterT7Available SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS chick islet1 [TJ#44]
Plasmid#16273DepositorInsertislet1 (ISL1 Chicken)
ExpressionBacterialAvailable SinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Ste7-EE
Plasmid#52681Purposeexpression of constitutively active Ste7 kinase in bacteriaDepositorInsertSte7 (STE7 Budding Yeast)
TagsGST and MycExpressionBacterialMutationS359E/T363E constitutively activePromotertacAvailable SinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
Tags6xHis tag and DsbA signal sequence for periplasmiā¦ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylatā¦PromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR PKAKTRn-Clover
Plasmid#177199PurposeGateway entry vector encoding PKAKTRnew-CloverDepositorInsertPKAKTRnew-mClover
ExpressionBacterialAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBMT-4
Plasmid#22837DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
PTPN11
Plasmid#38965PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
M13cp-dg1 hp
Plasmid#218093PurposeHelper plasmid that allows phagemid production upon complementation with a phagemid expressing pIDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3521 pET ubcH6
Plasmid#8653DepositorInsertUbcH6 (UBE2E1 Human)
ExpressionBacterialAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHCKAn-phoAp-GRFT
Plasmid#158747Purposelow phosphate phoAp induction of GriffithsinDepositorInsertphoAp Griffithsin
ExpressionBacterialPromoterphoAAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBZcsx27
Plasmid#89900PurposeExpresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx27
ExpressionBacterialPromoterLacAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #2
Plasmid#104205PurposeBacterial expression of WW domain #2 from NEDD4LDepositorInsertNEDD4L WW domain #2 (NEDD4L Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHYDuet183
Plasmid#41685DepositorInsertPhoRadA intein
TagsGB1 and His-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK506
Plasmid#155382PurposedCas12a (D917A) + PA4-mVenus. (removed crRNAs)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
UseCRISPRExpressionBacterialMutationD917A (nuclease-deactivating)Available SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'-t7- YFP
Plasmid#186564PurposepMB1' ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(D181N)
Plasmid#99643PurposeBacterial expression of RagA-RagC(D181N)DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBY2_ligD(FnCpf1)
Plasmid#104623PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligDDepositorInsertcrRNA to ligD
UseCRISPRExpressionBacterialPromoterPj23119Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107576PurposeB. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic BiologyTagsB. megaterium DSM319 xylA leader sequence (MTSSKIā¦ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterpdh promoter Bacillus megaterium DSM319Available SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-merR_O
Plasmid#127023PurposeBasic Part O- ORF; merR; transcription factorDepositorInsertPart merR_o
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only