30,808 results

1. SARS-CoV-2_N_deltaNTD

   Plasmid

   #157869

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   deltaNTD

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB1-sfGFP

   Plasmid

   #103983

   Purpose

   Plasmid encoding sfGFP

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   sfGFP
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S30R, Y39N, …

   Promoter

   proC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS1929

   Plasmid

   #205968

   Purpose

   JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH crRNA
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-TRP120

   Plasmid

   #71732

   Purpose

   inducible expression of TRP120 in E. coli

   Depositor

   Jere McBride

   Article

   Luo et al Clin Vaccine Immunol. 2009 Jul;16(7):982

   Insert

   TRP120
   

   Tags

   6xHIS and Thioredoxin

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAB085d

   Plasmid

   #79209

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ptet

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. NylB_pET29b

   Plasmid

   #215414

   Purpose

   Expression construct for NylB gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB
   

   Expression

   Bacterial

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. S9_attL1-EGFP-C-term-attL2

   Plasmid

   #128473

   Purpose

   EGFP entry clone

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   EGFP entry clone
   

   Expression

   Bacterial

   Available Since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. L1-H210Q

   Plasmid

   #15500

   Depositor

   Vance Lemmon

   Article

   Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

   Insert

   L1-H210Q (L1CAM Human)
   

   Expression

   Bacterial

   Mutation

   Histidine 210 changed to Glutamine

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

9. GUADA

   Plasmid

   #42382

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GUADA (GDA Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pJK_proK1_eGFP

    Plasmid

    #59853

    Purpose

    Constitutively expresses eGFP with proK1 promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    proK1

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCellFree_KA1_ZIKVC

    Plasmid

    #145339

    Purpose

    Cell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Capsid
    

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Promoter

    T7- CMV/CAG

    Available Since

    April 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SRSF9_2xRRM_pGEX

    Plasmid

    #135120

    Purpose

    Recombinant expression of SRSF9 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    SRSF9 (SRSF9 Human)
    

    Tags

    GST-SBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAN-PBAD-sgRNA-A2NT

    Plasmid

    #62257

    Purpose

    expression of A2NT sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A2NT sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_THRA_Hormone-recep

    Plasmid

    #109810

    Purpose

    Protein expression and purification of THRA_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    THRA_Hormone-recep (THRA Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPS0314

    Plasmid

    #115507

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMINTC3H

    Plasmid

    #110075

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLIParaF.Ec-250n

    Plasmid

    #18855

    Depositor

    Wolf Frommer

    Article

    Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

    Insert

    arabinose FRET sensor
    

    Tags

    CFP and Venus

    Expression

    Bacterial

    Available Since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pXWJ109AsAmp30E

    Plasmid

    #123144

    Purpose

    Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J109, ParsR, PhrpLE

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T1-MBP-TEV-NAP1 (WT)

    Plasmid

    #208871

    Purpose

    For expression in E. coli of NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-PaODH

    Plasmid

    #110802

    Purpose

    Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

    Insert

    Pa4835
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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