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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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33,128 results

Showing: 9841 - 9860 of 33128 results

  4. pVSL10

    Plasmid
    #201182
    Purpose
    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable
    Depositor
    Jack Szostak
    Article
    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.
    Insert
    Bst polymerase large fragment (BF) F710Y/D598A
    Tags
    HRV 3C protease cleavage site and His6
    Expression
    Bacterial
    Mutation
    F710Y D598A
    Promoter
    T5-lacO
    Available Since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201182-plasmid-map-sequence-id-406202

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  12. pMM7

    Plasmid
    #201213
    Purpose
    Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.
    Depositor
    Alex Nielsen
    Article
    Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1
    Inserts
    sfGFP
    Spo0A right flank
    Spo0A left flank
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    F11V, T13P, N39D, A179A
    Available Since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201213-plasmid-map-sequence-id-405310

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  15. pJYP1

    Plasmid
    #202406
    Purpose
    Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)
    Depositor
    Remy Loris
    Article
    Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    fdeA
    Available Since
    Aug. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202406-plasmid-map-sequence-id-403395

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  16. His-AgdNK

    Plasmid
    #203226
    Purpose
    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203226-plasmid-map-sequence-id-403332

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  20. pEXP5-NT/pT7 LasR

    Plasmid
    #193629
    Purpose
    Constitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LasR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 RNAP promoter
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193629-plasmid-map-sequence-id-382851

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Showing: 9841 - 9860 of 33128 results