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1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Publication

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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P-BT3332

Plasmid

#188110

Purpose

pBolux harboring the promoter region upstream of BT3332

Depositor

Guy Townsend

Publication

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT3332 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT3332

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUS252L

Plasmid

#191832

Purpose

Expresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbones

Depositor

Nicholas Coleman, Mark Somerville

Insert

fuBFP

Expression

Bacterial

Available since

Jan. 17, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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enz.083-NMT1

Plasmid

#154047

Purpose

Expresses NMT1 in E. coli

Depositor

Christopher Voigt

Publication

Wallace et al Advanced Functional Materials 2020

Insert

NMT1 (N-myristoyltransferase 1) (NMT1 Human)

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pXD69m1-TetW

Plasmid

#191246

Purpose

E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD69m2-AphA

Plasmid

#191247

Purpose

E. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

Plasmid

#191627

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

Plasmid

#191650

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

Plasmid

#192273

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mTagBFP2

Plasmid

#191746

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Publication

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mTagBFP2

Expression

Bacterial

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET29-hMIGA2(170-593)-6xhis

Plasmid

#192834

Purpose

Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

Depositor

Karin Reinisch

Publication

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

6xhis

Expression

Bacterial

Promoter

T7

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJLG029

Plasmid

#192980

Purpose

BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification

Depositor

Clay Fuqua

Publication

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

pruR-3xFLAG

Tags

3XFLAG

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE075

Plasmid

#176832

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

714

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEE060

Plasmid

#176817

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

611

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAM2314-RbcS-mNG

Plasmid

#165582

Purpose

Cyanobacterial carboxysome reporter with mNeonGreen driven by native rbcS promoter

Depositor

Daniel Ducat

Publication

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

mNeonGreen

Expression

Bacterial

Promoter

Prbcs

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-131

Plasmid

#185923

Purpose

Himar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein gene

Depositor

Mitja Remus-Emsermann

Publication

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mTurquoise2

Expression

Bacterial

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pPbuCas13b

Plasmid

#184565

Purpose

Inducible expression of PbuCas13b nuclease in bacteria.

Depositor

Chase Beisel

Publication

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Prevotella buccae

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSA12

Plasmid

#186546

Purpose

LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSA12

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-G65S/Y203I/Q204E/E222D/R223F

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAB213

Plasmid

#101677

Purpose

mCherry under T7 promoter control

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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