31,786 results
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Plasmid#22213DepositorAvailable SinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only
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pMV306hsp+FFluc
Plasmid#26156DepositorInsertFirefly luciferase
ExpressionBacterialMutationFirefly luciferase gene codon optimised for mycob…Available SinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7RNAP
Plasmid#74096PurposeT7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNADepositorInsertT7 RNAP
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
SUMO-PNGaseF
Plasmid#228498Purpose10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)DepositorInsertngl
Tags10xHis-SUMO(Smt3)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS8 AA 1-308
Plasmid#196905PurposeExpresses His-tagged Drosophila IntS8 amino acids 1-308DepositorAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmRFP_LAP2beta_IRES_puro2b
Plasmid#21047DepositorAvailable SinceJuly 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
HA-AR-LBD
Plasmid#171239PurposeMammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)DepositorInsert1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-Histag-Avidin
Plasmid#214836PurposeThis plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-Histag-Avidin
ExpressionBacterialPromoterT7Available SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19 Giles integrase
Plasmid#170049PurposepUC19 with Giles integraseDepositorInsertGIles Integrase
ExpressionBacterialAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-MacroGreen
Plasmid#160665PurposeExpresses GFP fusion of a multisite mutant of the Af1521 proteinDepositorInsertAf1521 modified synthetic construct eGFP fusion
TagseGFP and hexahistidine_TEV recognitionExpressionBacterialPromoterLacIAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFosill-1
Plasmid#89695Purposeto make Fosmids that can be converted into Illumina sequencable librariesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Blue
Plasmid#220782PurposeExpresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertblue chromoprotein (aeBlue)
Tags8xHisExpressionBacterialAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
Tags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV306DIhsp+LuxG13
Plasmid#49999PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertBacterial luciferase operon + G13 promoter
ExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterG13 from M. marinum G13 promoter driving luxCAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-PfuX7
Plasmid#182364PurposeExpresses PfuX7 DNA polymeraseDepositorInsertPfuX7 DNA polymerase
TagsHis-tagExpressionBacterialMutationV93QPromoterT7Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
ExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-GFPnanobody
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAJM.773
Plasmid#108527PurposeVanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.DepositorInsertVanRAM + PVanCC-YFP
ExpressionBacterialAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-GroEL/ES
Plasmid#83922PurposeCo-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.DepositorInsertsSkp
Gro EL/ES
ExpressionBacterialPromoterT7Available SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only