30,808 results

1. pBluescript SK +/- mTRF1

   Plasmid

   #12303

   Depositor

   Titia de Lange

   Article

   Broccoli et al Hum Mol Genet. 1997 Jan . 6(1):69-76.

   Insert

   mTRF1 (Terf1 Mouse)
   

   Expression

   Bacterial

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

2. Ubiquitin G76C

   Plasmid

   #86583

   Purpose

   Bacterial expression of Ubiquitin G76C for cross linking studies

   Depositor

   Sharon Rozovsky

   Article

   Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

   Insert

   Ubiquitin (UBC Human)
   

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   TEV site; G76C

   Promoter

   Tac

   Available Since

   Feb. 24, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET15-EF1p_mFAP2b

   Plasmid

   #159469

   Purpose

   Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

   Depositor

   David Baker

   Insert

   EF1p_mFAP2b
   

   Tags

   6xHis-thrombin-TEV

   Expression

   Bacterial

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNSSCPG

   Plasmid

   #183187

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   superfolder GFP
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29-hMIGA2(170-593)-6xhis

   Plasmid

   #192834

   Purpose

   Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p045

   Plasmid

   #70057

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with pDC184

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   C2AB-linker-SNAP-25_C
   SNAP-25_N
   

   Tags

   No tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP12068

   Plasmid

   #222356

   Purpose

   Tn7 integrative vector with PabstBR promoter expression cassette and kanR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

   Depositor

   Jason Peters

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

   Plasmid

   #204798

   Purpose

   Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   West Nile NS2B-NS3 fusion
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGL001

   Plasmid

   #115674

   Purpose

   Fluorescent reporter for E.coli K12 fliC promoter activity

   Depositor

   Gene-Wei Li

   Article

   Parker et al J Bacteriol. 2019 Sep 6;201(19). pii: JB

   Insert

   fliC promoter fused with GFP
   

   Tags

   Green fluorescent protein (GFP)

   Expression

   Bacterial

   Promoter

   fliC promoter

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_VCP_AAA

    Plasmid

    #109964

    Purpose

    Protein expression and purification of VCP_AAA

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    VCP_AAA (VCP Human)
    

    Expression

    Bacterial

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJUMP48-2A(sfGFP)

    Plasmid

    #126993

    Purpose

    Level 2 vector. sfGFP reporter. Origin pUC (high copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22b-BirA-CPDSalI

    Plasmid

    #38258

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    birA (birA E. coli)
    

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pGEX4T1-BirA

    Promoter

    T7

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

13. ALARM NMR

    Plasmid

    #90209

    Purpose

    Expresses the human La antigen for use in ALARM NMR

    Depositor

    Michael Walters

    Article

    Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi:

    Insert

    La antigen (SSB Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    contains amino acids 100-324

    Promoter

    T7 promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. phFluc-WT

    Plasmid

    #177714

    Purpose

    Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

    Depositor

    Erik Böttger

    Article

    Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

    Insert

    hFluc WT
    

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGex-6p2_ATRX

    Plasmid

    #221377

    Purpose

    Expresses ATRX in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Transcriptional regulator ATRX (ATRX Human)
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. prp8-G736E

    Plasmid

    #108450

    Purpose

    prp8-G736E in pRS313 backbone for expression in yeast

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    changed G736 to E

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB355

    Plasmid

    #22070

    Depositor

    Emmanuelle Bouveret

    Article

    Gully et al Proteomics. 2006 Jan . 6(1):282-93.

    Type

    Empty backbone

    Tags

    T18

    Expression

    Bacterial

    Available Since

    Oct. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a_TIAL1

    Plasmid

    #106092

    Purpose

    bacterial protein expression of TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIAL1 (TIAL1 Human)
    

    Tags

    6xHis-thrombin and T7

    Expression

    Bacterial

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. H14-MBP-SUMO-Cys-Linker-BAF_G47E

    Plasmid

    #101779

    Purpose

    Expresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Tags

    His14-MBP-SUMO

    Expression

    Bacterial

    Mutation

    G47E mutation

    Promoter

    T5

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAJM.1459

    Plasmid

    #108521

    Purpose

    P3B5C-YFP Reporter. DHBA sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    P3B5C-YFP
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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