30,808 results

1. pBA2257

   Plasmid

   #38151

   Depositor

   David Waugh

   Article

   Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

   Insert

   MBP-proMeCPA fusion protein
   

   Tags

   maltose binding protein

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. MERS-coV-NSP3-c001

   Plasmid

   #173085

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   D1109-D1275, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Fer-SH2

   Plasmid

   #46425

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Fer (FER Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 450-558

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXY1

   Plasmid

   #90487

   Purpose

   Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

   Depositor

   Sheng Yang

   Article

   Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. H2AFY2

   Plasmid

   #39052

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   H2AFY2 (MACROH2A2 Human)
   

   Tags

   His6-Thioredoxin-TEV

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pGST2-ALIX (795-811)

   Plasmid

   #21502

   Depositor

   James Hurley

   Article

   Lee et al Science. 2008 Oct 24. 322(5901):576-80.

   Insert

   ALIX (PDCD6IP Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pAAH5

   Plasmid

   #41829

   Purpose

   Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Expression

   Bacterial and Yeast

   Promoter

   T7,TEF

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFAB3677

   Plasmid

   #47833

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 2 and BCD1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   see insert

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCD315

   Plasmid

   #113321

   Purpose

   pBAD-W108 scRNA.b1

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   scRNA.b1_MS2
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET15b-hnRNP-F

    Plasmid

    #23021

    Depositor

    Douglas Black

    Article

    Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

    Insert

    hnRNP-F (HNRNPF Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

11. MBP-RGG-SmBit-RGG

    Plasmid

    #176644

    Purpose

    SmBit fused to LAF-1 RGG domain with N-terminal MBP tag

    Depositor

    Daniel Hammer

    Article

    Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

    Insert

    MBP-RGG-SmBit-RGG
    

    Expression

    Bacterial

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-32a(+)-TGFB3m

    Plasmid

    #120289

    Purpose

    Produce human recombinant mTGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    monomeric Transforming Growth Factor-β3
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM-T Dmel Or9a

    Plasmid

    #59637

    Purpose

    Probe for Drosophila melanogaster Or9a expression

    Depositor

    Richard Axel, Leslie Vosshall

    Article

    Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

    Insert

    Or9a (Or9a Fly)
    

    Expression

    Bacterial

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. GST-FUS 465-526

    Plasmid

    #44983

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (465-526aa) (FUS Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 465-526

    Promoter

    tac

    Available Since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pETM30-ARC105

    Plasmid

    #133426

    Purpose

    Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

    Depositor

    Gerhard Wagner

    Article

    Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

    Insert

    PCQAP (MED15 Human)
    

    Expression

    Bacterial

    Mutation

    Contains amino acids 5-78 fused to C-terminal of …

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pF712-pET28a-Hs-delta44-GCDH-NHis wt

    Plasmid

    #85113

    Purpose

    expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta44) GCDH (GCDH Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    silent SNP: G1041T

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

    Plasmid

    #165030

    Purpose

    Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBM3R1-B1

    Plasmid

    #74678

    Purpose

    BM3R1-B1 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSB1-BM3R1-PBM3R1-YFP
    

    Expression

    Bacterial

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET24a-ncOGT-TPR

    Plasmid

    #190822

    Purpose

    For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    C467X. Also codon optimized for expression in E.c…

    Promoter

    T7

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pET-NCBD + ACTR

    Plasmid

    #99335

    Purpose

    Bacterial coexpression of CBP NCBD and NCoA3 binding domains

    Depositor

    Peter Wright

    Article

    Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

    Inserts

    CBP NCBD (2059-2117) (CREBBP Mouse)
    SRC3 (1023-1093) (NCOA3 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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