33,128 results
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Plasmid#118584PurposeBacterial expression of E.coli DHFR N23PP and L28F mutantDepositorInsertDHFR
ExpressionBacterialMutationL28 mutated to F28, N23 mutated to P23, a proline…PromoterT7Available SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2
Plasmid#116953PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG10-pGlnA-mCherry
Plasmid#102467PurposeExpresses mCherry fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.DepositorArticleInsertmCherry
ExpressionBacterialPromoterpGlnAAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-H2B(1-114)_wt
Plasmid#118252PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNaDepositorInsertxlH2B(1-114)
TagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialPromoterT7Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpot2
Plasmid#117063PurposeFor expression of proteins with C-terminal Spot-Tag in E. coliDepositorTypeEmpty backboneTagsSpot-TagExpressionBacterialAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSPPc-Gm
Plasmid#116919PurposeGene constitutive expression from a Pc promoterDepositorTypeEmpty backboneExpressionBacterialPromoterPc promoter from Delftia acidovorans C17Available SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI
Plasmid#115214PurposeExpresses PauCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPauCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 UBXN2A
Plasmid#113505PurposeBacterial expression of GST-tagged UBXN2ADepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0253
Plasmid#115505PurposeSuitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.DepositorInsertRlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM166
Plasmid#84197PurposeDisturb the expression of miR166 in riceDepositorInsertmiR166
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM319
Plasmid#84201PurposeDisturb the expression of miR319 in riceDepositorInsertmiR319
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM159
Plasmid#84195PurposeDisturb the expression of miR159 in riceDepositorInsertmiR159
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG202
Plasmid#115503PurposeClone of a level 1 unit which encodes IPTG-inducible sfGFPDepositorInsertpL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B5-1
Plasmid#111480PurposeLeu-tRNA synthetaseDepositorInsertLeu-tRNA synthetase
Tags6xHisExpressionBacterialMutationI10KAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B3-4
Plasmid#111474Purposetranslational release factor 2DepositorInserttranslational release factor 2
Tags6xHisExpressionBacterialAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FPPS
Plasmid#108952Purposeheterologous expression of FPPS of G. stearothermophilus in E. coliDepositorInsertFarnesyldiphosphat synthase (FPPS)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIC-LexAGAD
Plasmid#112807Purposegateway cloning destination vector to express enhancer driven LexAGAD in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBADM11-HMGR
Plasmid#108946Purposeheterologous expression of HMGR of S. solfataricus in E. coliDepositorInsertHMG-CoA reductase (HMGR)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only