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pTn7·xylS

Plasmid

#122591

Purpose

Plasmid for genomic integraction of the xylS regulator into the Tn7 insertion site

Depositor

Pablo Ivan Nikel

Publication

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

xylS

Use

Synthetic Biology; Tn7 genomic integration

Expression

Bacterial

Promoter

Pm

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJMP1217

Plasmid

#119258

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO5.01

Plasmid

#121418

Purpose

pUC19_BsaI-dCas9-BsaI

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

dcas9

Expression

Bacterial

Promoter

lac

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP

Plasmid

#121676

Purpose

Bacterial expression of MBP fusion proteins

Depositor

Reinhard Grisshammer, Richard Henderson

Publication

Tucker et al Biochem J. 1996 Aug 1;317 ( Pt 3):891-9.

Insert

maltose binding protein

Expression

Bacterial

Promoter

lac promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-A2aTr316-H10

Plasmid

#121683

Purpose

Bacterial expression of adenosine A2a receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Publication

Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

Insert

adenosine A2a receptor (ADORA2A Human)

Tags

His10

Expression

Bacterial

Promoter

lac

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD010

Plasmid

#116851

Purpose

Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010

Depositor

Friedrich Simmel

Publication

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL
mScarletI

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Publication

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Brg1 Del1 pET28a

Plasmid

#122115

Purpose

Expression Brg1 amino acids 1-381 in bacteria

Depositor

Paul Herring

Publication

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET-SOMA

Plasmid

#118935

Purpose

Used to express the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Publication

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana

Expression

Bacterial

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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NmeCas9_3XNLS_pMCSG7

Plasmid

#120078

Purpose

Bacterial expression of wtNmeCas9 with 3X NLS

Depositor

Erik Sontheimer

Publication

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

NmeCas9

Expression

Bacterial

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-ETV6-ETS

Plasmid

#111180

Purpose

Minimal ETS domain of human ETV6 (TEL)

Depositor

Gregory Poon

Publication

Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

Insert

ETV6 ETS domain (ETV6 Mouse, Bovine, Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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BPK2013-AkCas12b

Plasmid

#121950

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Tags

10xHis tag and 3xHA tag

Expression

Bacterial

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRAN334

Plasmid

#120814

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

Depositor

Craig Ellermeier, David S. Weiss

Publication

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms

Expression

Bacterial

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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SRF WT pGEX4T

Plasmid

#121100

Purpose

Expression of SRF as a GST fusion protein

Depositor

Paul Herring

Publication

Herring et al J Biol Chem. 2001 Apr 27;276(17):14482-9

Insert

SRF (SRF Human)

Tags

GST

Expression

Bacterial

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMM1157

Plasmid

#112534

Purpose

pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

Depositor

Timothy Lu

Publication

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
luxCDABE

Expression

Bacterial

Promoter

PL(HrtO) and ProD

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL001

Plasmid

#117825

Purpose

Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_YlGUT1_YlCyc1TT

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1071

Plasmid

#119245

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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dFP-mini

Plasmid

#120543

Purpose

Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

Depositor

Brian Chow

Publication

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein mini (dFP-mini)

Tags

6xHis - TEV cleavage

Expression

Bacterial

Promoter

T7

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2_PresT-mCherryBb

Plasmid

#118238

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

resT promoter driven mCherry

Expression

Bacterial

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 Aqua toehold switch

Plasmid

#107356

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 8 + Aquamarine

Expression

Bacterial

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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