30,753 results

1. pJK438

   Plasmid

   #71590

   Purpose

   Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)

   Depositor

   T. J. Kappock

   Article

   Murphy et al Acta Crystallogr F Struct Biol Commun. 2

   Insert

   citrate synthase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK240

   Plasmid

   #71127

   Purpose

   Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   citrate synthase
   

   Tags

   GyrA intein and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET14b PHAS-I F113A

   Plasmid

   #15680

   Depositor

   John Lawrence

   Article

   Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

   Insert

   PHAS-I F113A (Eif4ebp1 Rat)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F113A mutation.

   Available Since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pMA7

   Plasmid

   #79967

   Purpose

   TM-MAGE strain

   Depositor

   Morten Sommer

   Article

   Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

   Inserts

   Lambda Red recombinase beta subunit
   DNA adenine methylase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV113

   Plasmid

   #63180

   Purpose

   pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX Bmx-SH2

   Plasmid

   #46409

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Bmx (BMX Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 286-400

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. C-BLa 1.1

   Plasmid

   #88997

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   C-BLa 1.1 fusion protein
   

   Tags

   Flag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS52

   Plasmid

   #104334

   Purpose

   Expresses sfGFP from the OmpR regulated PompF promoter.

   Depositor

   Jeffrey Tabor

   Article

   Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

   Insert

   superfolder GFP
   

   Expression

   Bacterial

   Promoter

   PompF

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p005kanRFP

   Plasmid

   #58533

   Purpose

   RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   Red fluorescent protein
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T5 between two lac operators

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYH71

    Plasmid

    #217381

    Purpose

    pTrc99A-mCherry-mcdB for bacterial expression

    Depositor

    Anthony Vecchiarelli

    Article

    Hoang et al Nat Commun. 2024 Apr 15;15(1):3222. doi:

    Insert

    mcdB
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28(+)-NudC

    Plasmid

    #175022

    Purpose

    Purification of NudC enzyme

    Depositor

    Jeppe Vinther

    Article

    Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

    Insert

    nudC
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQTEV-PRKRA

    Plasmid

    #31571

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbA4a-RFP

    Plasmid

    #35298

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    ProE

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDZ207 (loxP KAN loxP 24xPP7SL MDN1)

    Plasmid

    #35191

    Depositor

    Robert Singer

    Article

    Larson et al Science. 2011 Apr 22;332(6028):475-8.

    Insert

    loxP KAN loxP 24xPP7SL MDN1
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Available Since

    Feb. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS23: pHelper(PmcCAST)_entry_ΔTnsD

    Plasmid

    #168156

    Purpose

    Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB and TnsC)
    PmcTniQ
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. RP1B

    Plasmid

    #26563

    Depositor

    Wolfgang Peti

    Article

    Peti et al Protein Expr Purif. 2007 Jan . 51(1):1-1

    Type

    Empty backbone

    Tags

    His6, TEV cleavage site, and Thio-6

    Expression

    Bacterial

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

17. FTHFDB

    Plasmid

    #42339

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FTHFDB (ALDH1L1 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pQZ210

    Plasmid

    #106414

    Purpose

    Co-expresses trans SNARE complex with pDC183 in E.coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Inserts

    Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
    Syntaxin-1A fragment 191-256 (Stx1a Synthetic, Rat)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Fragment 191-256 , Codon optimization and Fragmen…

    Promoter

    T7

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His6 TEV co-transformation cloning vector (13S-B)

    Plasmid

    #48324

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTorPE-GEM-GECO1

    Plasmid

    #32463

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO1
    

    Use

    Lab constructed

    Tags

    6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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