30,744 results

1. pET His6 TEV co-transformation cloning vector (13K-B)

   Plasmid

   #48316

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a.MJ0285

   Plasmid

   #11304

   Depositor

   Sung-Hou Kim

   Article

   Kim et al J Struct Biol. 1998 Jan . 121(1):76-80.

   Insert

   small heat shock protein (MJ_0285 M. jannaschii)
   

   Expression

   Bacterial

   Available Since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-c5X-Darpin E_01

   Plasmid

   #66996

   Purpose

   Expresses malE-Darpin E_01 in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-Darpin E_01
   

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

   Plasmid

   #91861

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) R0179A/K1080A
   

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K1080A

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbS8k-RFP_R46W

   Plasmid

   #109415

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   R46W repA mutation to increase copy number

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SH3PXD2A-PX (1-126)

   Plasmid

   #119115

   Purpose

   Bacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SH3PXD2A-PX (1-126) (SH3PXD2A Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   May 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-51b(+)-Caprola_14

   Plasmid

   #194675

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_14
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-51b(+)-Caprola_15

   Plasmid

   #194676

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_15
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM579

   Plasmid

   #68882

   Purpose

   NanoLuc expressed constiutively from P1, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Insert

   NanoLuc
   

   Expression

   Bacterial

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. USP04

    Plasmid

    #25223

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    usp04.001.229 (Usp4 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pNCST-AausFP2

    Plasmid

    #129500

    Purpose

    Expresses AausFP2 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausFP2
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEC581

    Plasmid

    #174369

    Purpose

    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    RFP with PAM rich sequence upstream promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23117

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEC625

    Plasmid

    #174368

    Purpose

    Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-FAM86A

    Plasmid

    #85117

    Purpose

    bacterial expression of FAM86A

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EEF2KMT (FAM86A Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR6K-GFP-RFP

    Plasmid

    #199652

    Purpose

    R6K backbone with Golden Gate (GG) cloning sites

    Depositor

    IIya Finkelstein

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLS.416

    Plasmid

    #187207

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2)
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbB8k-csg-NbMS2

    Plasmid

    #171701

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

    Insert

    csgBACEFG, with nanobody fused to CsgA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTE1059

    Plasmid

    #186628

    Purpose

    Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.

    Depositor

    Eriko Takano

    Article

    Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

    Inserts

    scbA
    scbB
    scbC
    

    Use

    Synthetic Biology

    Tags

    6xHis and 6xHis-thrombin

    Expression

    Bacterial

    Promoter

    Synthetic promoter B10, Synthetic promoter B19, a…

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. L3MBTL2

    Plasmid

    #25244

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    L3MBTL2_14 (L3MBTL2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pJMP2836

    Plasmid

    #160674

    Purpose

    Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Insert

    gmc6 (gfp) sgRNA
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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