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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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31,943 results

Showing: 11341 - 11360 of 31943 results

  3. pSP019

    Plasmid
    #168195
    Purpose
    expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.
    Depositor
    James Fleckenstein
    Insert
    eatA[H134R]
    Expression
    Bacterial
    Mutation
    H134R mutation
    Promoter
    araBAD
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    168195-plasmid-map-sequence-id-339468

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  4. pET15-EF1p_mFAP2b

    Plasmid
    #159469
    Purpose
    Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1p_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159469-plasmid-map-sequence-id-319725

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  5. pNB3033

    Plasmid
    #113397
    Purpose
    Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible
    Depositor
    Suzanne Sandmeyer
    Insert
    Ty3 Gag3 codon optimized for E. coli expression
    Tags
    Gag3-6XHis tag
    Expression
    Bacterial
    Available Since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113397-plasmid-map-sequence-id-217374

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  6. GOT1

    Plasmid
    #39022
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GOT1 (GOT1 Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid
    #181787
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181787-plasmid-map-sequence-id-354706

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  10. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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Showing: 11341 - 11360 of 31943 results