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pET28a-HsSENP3(307-574)

Plasmid

#71481

Purpose

Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Deleted amino acids 1-306 (catalytic domain only)

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
pgRNA-guideless

Plasmid

#129464

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Guideless gRNA backbone

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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SRS-MTBD(22/19), AB(monomer), wt

Plasmid

#22380

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

His and myc

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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TIAM1_PDZ_1

Plasmid

#103925

Purpose

Protein expression and purification of TIAM PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TIAM1 (TIAM1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-ORF3b (S23Q)

Plasmid

#165088

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)

Expression

Bacterial

Mutation

Stop codon to glutamine at 23rd amino acid

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTn7·xylS

Plasmid

#122591

Purpose

Plasmid for genomic integraction of the xylS regulator into the Tn7 insertion site

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

xylS

Use

Synthetic Biology; Tn7 genomic integration

Expression

Bacterial

Promoter

Pm

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Article

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Tags

His and thrombin cleavage

Expression

Bacterial

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
TSTA3A-c013

Plasmid

#53663

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

TSTA3A

Tags

His6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pYUB28b-MBP

Plasmid

#89178

Purpose

mycobacteria shuttle vector

Depositor

Ted Baker, Ghader Bashiri

Article

Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Type

Empty backbone

Use

Mycobacteria shuttle vector

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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VGFR1_HUMAN_D0

Plasmid

#79743

Purpose

This plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VGFR1

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pBS IRES EGFP (DM#271)

Plasmid

#15080

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

IRES EGFP

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pQcre1

Plasmid

#135659

Purpose

Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

ppag promoter

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA5

Plasmid

#64985

Purpose

Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBS mScx (CT#404)

Plasmid

#13958

Depositor

Cliff Tabin

Article

Schweitzer et al Development. 2001 Oct . 128(19):3855-66.

Insert

Scleraxis (Scx Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
C2-66 pComb3xss anti-cFos nanobody

Plasmid

#145833

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-66 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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T7-U5 (long)

Plasmid

#111337

Purpose

for in vitro snRNA transcription

Depositor

Christine Guthrie

Article

Ghetti et al RNA. 1995 Apr;1(2):132-45.

Insert

snR7-L

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-dDrp1-StrepII

Plasmid

#174421

Purpose

Expresses D. melanogaster Drp1 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1 (Drp1 Fly)

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKP968

Plasmid

#167653

Purpose

Express colicinB-His6, D17C, M261C

Depositor

Kathleen Postle

Insert

cba-his6 D17C, M261C

Expression

Bacterial

Mutation

cba-his6 with D17C, M261C mutations

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pYTK070

Plasmid

#65177

Purpose

Encodes ConR4 as a Type 5 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConR4

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-Spastin(87-616)DeltaExon4

Plasmid

#128794

Purpose

Bacteiral expression of Spastin M87 varient without exon 4

Depositor

Chris Hill

Article

Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

Insert

Spastin (SPAST Human)

Use

`

Tags

GST

Expression

Bacterial

Mutation

residues 87-616 without exon 4.

Promoter

T7

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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