30,738 results

1. pETM6-M.accABCD

   Plasmid

   #66865

   Purpose

   Encodes accA, accB, accC, accD in monocistronic configuration.

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   accA
   accB
   accC
   accD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUdO4t

   Plasmid

   #210249

   Purpose

   low-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDFNKI-his-3C-LIC-strep

   Plasmid

   #108705

   Purpose

   Expression of a His-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   His-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEM-T Dmel Or43b

   Plasmid

   #59654

   Purpose

   Probe for Drosophila melanogaster Or43b expression

   Depositor

   Leslie Vosshall, Richard Axel

   Article

   Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

   Insert

   Or43b (Or43b Fly)
   

   Expression

   Bacterial

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pBAD-rxRFP1.2

   Plasmid

   #67839

   Purpose

   redox-sensitive red fluorescent protein rxRFP1.2

   Depositor

   Huiwang Ai

   Article

   Fan et al Anal Bioanal Chem. 2016 Jan 12.

   Insert

   rxRFP1.2
   

   Tags

   His Tag

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

6. 4P7A

   Plasmid

   #61954

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MLH1:JMC01L-F09:C205690 (MLH1 Human)
   

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   March 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pWT037a

   Plasmid

   #107896

   Purpose

   W2.1 plasmid. Contains PTetO-SD8-BE2, PLacO-sgRNA1 and is part of CAMERA 2.1

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PTetO-SD8-BE2, PLacO-sgRNA1
   

   Expression

   Bacterial

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVRb03_up1198

   Plasmid

   #49706

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   sfgfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Pecf03_up1198

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPS2966

   Plasmid

   #8925

   Depositor

   Pamela Silver

   Article

   Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

   Insert

   NDR1 (STK38 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

10. MAGEA4

    Plasmid

    #39001

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAGEA4 (MAGEA4 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmMaple2-CAM

    Plasmid

    #101149

    Purpose

    Fluorescent protein for superresolution imaging

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    E. coli codon optimized mMaple2
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHH0103_SNX9-1/1

    Plasmid

    #91513

    Purpose

    Protein expression and purification of human SH3 domain construct SNX9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SNX9-1/1 (SNX9 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Pk-SOD1

    Plasmid

    #25574

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pk-SOD1
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 10, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pAVA-LGF2-Gal11p

    Plasmid

    #127481

    Purpose

    Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2-Gal11p (GAL11 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAVA

    Plasmid

    #127464

    Purpose

    AVA-seq plasmid with duel BstXI sites

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid

    #127483

    Purpose

    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    One nucleotide inserted between lambda CI and LGF…

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAVA-Gal11p-LGF2(fs)

    Plasmid

    #127482

    Purpose

    Negative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    Gal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    LGF2 is frame shifted by the insert of one nucleo…

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBORF-DBD

    Plasmid

    #127463

    Purpose

    ORF filtering plasmid (lambda cI associated plasmid for pAVA)

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

    Plasmid

    #183077

    Purpose

    Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and crRNA ending with a HDV ribozyme
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSL4672 (pEffector_TnpB3,ωRNA)

    Plasmid

    #210872

    Purpose

    Expresses GstTnpB3 and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstTnpB3
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge