30,736 results

1. pJB089

   Plasmid

   #98914

   Purpose

   Express Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coli

   Depositor

   Jie Xiao

   Article

   Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

   Inserts

   FtsZ
   ZapA
   

   Tags

   Dronpa and pAmCherry1

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL0746 (pDonor_R-MmeI)

   Plasmid

   #130646

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (R*)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   TGTTGATA --> TGTTGGAA

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL90-Nat-2xHO

   Plasmid

   #139069

   Purpose

   CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Insert

   Two guide RNAs targeting the HO endonuclease
   

   Expression

   Bacterial and Yeast

   Promoter

   TDH3

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD/HisB (TIRSTD)-sfGFP

   Plasmid

   #189674

   Purpose

   titratable expression of sfGFP in bacteria

   Depositor

   Daniel Daley

   Article

   Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

   Insert

   sfGFP
   

   Tags

   His-T7-EK

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRB-polySH33

   Plasmid

   #175233

   Purpose

   Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. RPS6KA1

   Plasmid

   #39021

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RPS6KA1 (RPS6KA1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pRSFDuet-1_VNp-StefinA

   Plasmid

   #182403

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-stefin (CSTA Human)
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-link-PAmCherry-FLAG:cat

   Plasmid

   #211832

   Purpose

   Plasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.

   Depositor

   Peng Chen

   Insert

   PAmCherry-FLAG-cat
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_DST-1/1

   Plasmid

   #91288

   Purpose

   Protein expression and purification of human SH3 domain construct DST-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   DST-1/1 (DST Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500

    Plasmid

    #92317

    Purpose

    Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    p70a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184738

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5407 pClpB

    Plasmid

    #1235

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    ClpB
    

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

13. M63 pPET28a-TEV-human beta cat domain C(666-781)

    Plasmid

    #17204

    Depositor

    Randall Moon

    Insert

    beta catenin (CTNNB1 Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    carboxy-terminal residues 666-781

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

14. K560_AviN_coBirA

    Plasmid

    #129766

    Purpose

    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    Avi-tag GLNDIFEAQKIEWH and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. DIRAS3

    Plasmid

    #55686

    Purpose

    expression of human DIRAS3

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    DIRAS3 (DIRAS3 Human)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

16. S2-77 pComb3xss anti-cFos nanobody

    Plasmid

    #145834

    Purpose

    Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-cFos (Human) recombinant llama nanobody S2-77 (FOS L. glama (llama))
    

    Tags

    6xHis-HA

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBsaGSCYP101A1

    Plasmid

    #108206

    Purpose

    CYP101A1

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

    Insert

    CYP101A1
    

    Expression

    Bacterial

    Promoter

    lac promoter

    Available Since

    April 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVRc03_1198

    Plasmid

    #49730

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS03_1198
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVRc22_4450

    Plasmid

    #49741

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS22_4450
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFLIPmal-40uDelta1YFP

    Plasmid

    #18853

    Depositor

    Wolf Frommer

    Article

    Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

    Insert

    optimized maltose FRET sensor
    

    Tags

    CFP and YFP

    Expression

    Bacterial

    Mutation

    N227A;

    Available Since

    Oct. 23, 2008

    Availability

    Academic Institutions and Nonprofits only