31,943 results

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TOPO iRFP670

Plasmid

#68443

Purpose

TOPO construct expressing iRFP670 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

iRFP670

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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AP575-1

Plasmid

#71277

Purpose

Expresses 3Xflag::tagRFP::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagRFP

Tags

3xFlag and myc

Expression

Bacterial

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEB2-mNeptune2

Plasmid

#104012

Purpose

Plasmid encoding mNeptune2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mNeptune2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (MSKGEE LIKENM… R32…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_EIF4E_IF4E

Plasmid

#110010

Purpose

Protein expression and purification of EIF4E_IF4E

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EIF4E_IF4E (EIF4E Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET24a-LaM4-3xTS

Plasmid

#162777

Purpose

Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Article

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope

Tags

BAP, HA, His6, T7, and TS site from proCCK

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBA789

Plasmid

#186244

Purpose

soc-F HR Donor Vector (T4 phage editing)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

BBR1 backbone + T4 phage homology

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Yes-SH2

Plasmid

#46532

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Yes (YES1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 152-261

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pVRa22_4450

Plasmid

#49676

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF22_4450

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2-LSSmOrange

Plasmid

#118856

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2-LSSmOrange

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.719

Plasmid

#108520

Purpose

PTtg-YFP Reporter. Nar sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTtg-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.711

Plasmid

#108512

Purpose

PPhlF-YFP Reporter. DAPG sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PPhlF-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXR1AtR2B2NtS1

Plasmid

#160879

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: pBAD, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX C_K nsp1 CoV2

Plasmid

#175516

Purpose

Expresses C_K for bioconjugation on nsp1

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

C_K nsp1 CoV2

Tags

GST tag

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPro18-cm

Plasmid

#17807

Depositor

Jay Keasling

Article

Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
pNCST-AausFP2

Plasmid

#129500

Purpose

Expresses AausFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-Darpin E_01

Plasmid

#66996

Purpose

Expresses malE-Darpin E_01 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Darpin E_01

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAV113

Plasmid

#63180

Purpose

pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pSiMPlh_N + pSiMPlh_C

Plasmid

#134316

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

hygR_gp41-1 N-intein + hygR_gp41-1 C-intein

Expression

Bacterial

Mutation

P254Q in hygR_gp41-1 N-intein

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pF712-pET28a-Hs-delta44-GCDH-NHis wt

Plasmid

#85113

Purpose

expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta44) GCDH (GCDH Human)

Tags

6xHis

Expression

Bacterial

Mutation

silent SNP: G1041T

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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B6-3

Plasmid

#111487

Purpose

Ser-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Ser-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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