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mCherryS131C

Plasmid

#48732

Purpose

Expresses mCherryS131C in E. coli cells

Depositor

Bradley Olsen

Article

Thomas et al ACS Nano. 2011 Jul 26;5(7):5697-707. doi

Insert

mCherryS131C

Tags

6xHis

Expression

Bacterial

Mutation

changed serine 131 to cysteine

Promoter

T5

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pET26b(+)-ATI_QconCAT_1

Plasmid

#163957

Purpose

Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

Depositor

Stefan Tenzer

Article

Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

Insert

ATI QconCAT

Tags

polyhistidine tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJOE3075.3

Plasmid

#135084

Purpose

L-rhamnose-inducible expression vector

Depositor

Josef Altenbuchner

Article

Wegerer et al BMC Biotechnol. 2008 Jan 14;8:2. doi: 10

Insert

P-rhaP-BAD-malE

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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ZIKV_Trigger_32B

Plasmid

#75009

Purpose

Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_32B

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX Hck-SH2

Plasmid

#46445

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Hck (HCK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 134-254

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pET15b-Casp8 delta DED C360A

Plasmid

#11828

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 8 minus Death Effecter Domain catalytic mutant (CASP8 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Minus Death Defecter Domain (aa 1-216), C360A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
M62 pPET28a-TEV-human beta cat domain N(1-137)

Plasmid

#17203

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Tags

HIS

Expression

Bacterial

Mutation

amino-terminal residues 1-137

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
9 pCOLI_A_C_GST

Plasmid

#160245

Purpose

C-term GST uncleavable Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pRM2

Plasmid

#8486

Depositor

Nancy Craig

Article

McKown et al J Bacteriol 1988 Jan;170(1):352-8.

Insert

attTn7 (tnsA Bacteria)

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4/2

Plasmid

#71712

Purpose

Membrane scaffold protein (MSP), half of helix 4 truncated

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pKM343

Plasmid

#71487

Purpose

Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-cat-loxP

Use

Source of loxp-hyg-cat- loxp for pcr amplificatio…

Expression

Bacterial

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAGE2.0-i

Plasmid

#194639

Purpose

Empty pAGE2.0 plasmid with pBAD inducible promoter

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/CURT_fluoA

Plasmid

#117998

Purpose

Expression of CURT_fluoA controlled by the ARA promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_fluoA

Expression

Bacterial

Promoter

ARA

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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MP-S2

Plasmid

#69691

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 AAG(Y127I-H136L)

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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MP-S3

Plasmid

#69692

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 d80-AAG(Y127I-H136L)

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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AD-003

Plasmid

#25502

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

AD-003 (NTMT1 Human)

Tags

6x His and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
Intersectin I SH3 A domain (human)

Plasmid

#47413

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain A (ITSN1 Human)

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain A

Promoter

tac

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pBbdCas9S(-10TC)_Psyn-sgRNA500

Plasmid

#149583

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-MPHOSPH8 Chromo

Plasmid

#59694

Purpose

Contains histone modification interacting domain (MPHOSPH8 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

MPHOSPH8 Chromo (MPHOSPH8 Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only

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His-NKX3.1

Plasmid

#42896

Depositor

Edward Gelmann

Article

Ju et al Biochemistry. 2009 Nov 10;48(44):10601-7

Insert

NKX3.1 (NKX3-1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only