32,967 results
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Plasmid#194591PurposeBacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertC7 (S )
ExpressionBacterialAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-jRCaMP1b
Plasmid#191468PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent proteinDepositorInsertjRCaMP1b
TagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExp-RBD-CHis
Plasmid#195002PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAurora
Plasmid#190158PurposeRegulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneTagsHis6 tagExpressionBacterialAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS2B
Plasmid#145344PurposeCell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFPDepositorInsertZIKV NS2B
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS4B
Plasmid#145347PurposeCell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFPDepositorInsertZIKV NS4B
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-HsPABPC1_J
Plasmid#146642PurposeBacterial Expression of HsPABPC1DepositorInsertHsPABPC1 (PABPC1 Human)
ExpressionBacterialMutationtwo silent mutations compared to the sequence giv…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-PfAgo_F
Plasmid#146265PurposeBacterial Expression of PfAgoDepositorInsertPfAgo
ExpressionBacterialMutationone silent T1704C and one non silent mutation A90…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-PfAgo_F
Plasmid#146270PurposeBacterial Expression of PfAgoDepositorInsertPfAgo
ExpressionBacterialMutationone non silent mutation A907G (I303V) and one sil…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
P-BT1763
Plasmid#188111PurposepBolux harboring the promoter region upstream of BT1763DepositorInsertThe B. thetaiotaomicron BT1763 promoter cloned into pBolux
ExpressionBacterialPromoterBT1763Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-TPR
Plasmid#190822PurposeFor expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags6x HisExpressionBacterialMutationC467X. Also codon optimized for expression in E.c…PromoterT7Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ
Plasmid#191628PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZDepositorInsertlacZ
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA-G83I
Plasmid#191239PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
Tagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and Glycin…PromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
Tagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192788PurposeaGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody enhancer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)
Plasmid#191801PurposeFull length wt AtPCO4 with Nt-His tagDepositorInsertAtPCO4-1
Tags6xHis, TEV cleavage siteExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_iRFP670
Plasmid#191749PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and iRFP670ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only