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MBTD1

Plasmid

#25329

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

MBTD1_5 (MBTD1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 130-566

Available Since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
SNX5-PX (26-170)

Plasmid

#119086

Purpose

Bacterial expression of human phox homology (PX) domain, SNX5-PX (26-170)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX5-PX (26-170) (SNX5 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSL0747 (pDonor_L-MmeI)

Plasmid

#130647

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (L*)

Use

CRISPR; Transposon

Expression

Bacterial

Mutation

TGTTGATG --> TGTTGGAG

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+-rasmKate

Plasmid

#105938

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

Insert

Ras

Tags

mKate

Expression

Bacterial

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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PAHA

Plasmid

#74663

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PKU1 (PAH Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP3(307-574)

Plasmid

#71481

Purpose

Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Deleted amino acids 1-306 (catalytic domain only)

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
TIAM1_PDZ_1

Plasmid

#103925

Purpose

Protein expression and purification of TIAM PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TIAM1 (TIAM1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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FabF (KASII) pet28 N-terminal His Tag

Plasmid

#75017

Purpose

Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

FabF (fabF E. coli)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only
pHis8-4b-SaDAH

Plasmid

#140128

Purpose

Expresses SaDAH with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase from Sinomenium acutum

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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PC4_3spc34h

Plasmid

#70700

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Tags

his-tag on C-terminus of spc34

Expression

Bacterial

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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Pk-SOD1

Plasmid

#25574

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

Pk-SOD1

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2010

Availability

Industry, Academic Institutions, and Nonprofits
T7 promoter + rpsL reporter plasmid

Plasmid

#156455

Purpose

A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

rpsL
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

tac and tet

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS3

Plasmid

#145355

Purpose

Cell-free expression vector for Zika virus NS3 protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Tags

mCherry and 8xHis

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSKA397

Plasmid

#80380

Purpose

mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

Depositor

Mustafa Khammash

Article

Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

Inserts

cat
metE

Use

Synthetic Biology; Mini-tn7 delivery vector

Expression

Bacterial

Promoter

cat promoter and cpcG2Δ59

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBbA19a-RFP

Plasmid

#126101

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-sr1(SIRPT1)

Plasmid

#75297

Purpose

Expresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 84-339

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse eIF2C3

Plasmid

#50355

Purpose

Mouse eIF2C3 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C3 (Ago3 Mouse)

Expression

Bacterial

Promoter

T3

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pYUB28b-MBP

Plasmid

#89178

Purpose

mycobacteria shuttle vector

Depositor

Ted Baker, Ghader Bashiri

Article

Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Type

Empty backbone

Use

Mycobacteria shuttle vector

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP-HMGB1-K184Rfs

Plasmid

#194544

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Bacterial

Mutation

K184Rfs*44

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only