30,420 results

1. pES001 MBP-GSK3β-HA-His

   Plasmid

   #154010

   Purpose

   Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Tags

   HA tag, His6, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pProEx-HTa-SOD1 WT

   Plasmid

   #191849

   Purpose

   express wild-type SOD1 in bacterial cells

   Depositor

   Nam-Chul Ha

   Article

   Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJMP3653

   Plasmid

   #222348

   Purpose

   Expression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

   Depositor

   Jason Peters

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETNb-cALFA

   Plasmid

   #159989

   Purpose

   For the protein expression with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB6 (CK2alpha')

   Plasmid

   #27084

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha' (CSNK2A2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23a-H6-TEV-cSrc

   Plasmid

   #214233

   Purpose

   Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

   Depositor

   Neel Shah

   Article

   Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

   Insert

   cSrc Kinase domain (SRC Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKCyR8

   Plasmid

   #149494

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCymRC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

   Plasmid

   #169161

   Purpose

   To express nsp10-nsp14 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZac2.1 gfaABC1D-LifeAct-GFP

   Plasmid

   #190199

   Purpose

   Expresses the small actin binding peptide, LifeAct, with a GFP reporter

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   LifeAct-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSG 25

    Plasmid

    #63867

    Purpose

    Expression of beta-galactosidase at high levels in E. coli

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtacI

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only