32,417 results
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Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
ExpressionBacterialPromoterT7Available SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Dp
Plasmid#79159PurposeExpresses the Dp biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Dp
ExpressionBacterialPromoterT7Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJP_Osc05
Plasmid#229040PurposeLight inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.DepositorInsertpBAD promoter
ExpressionBacterialAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-cAMP
Plasmid#225249PurposeBacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
ExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9_CL
Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyExpressionBacterialPromoterEc-TTL-P112 and Ec-TTL-P104Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEvoltac-EcW-TGA-h14
Plasmid#218764Purposeencodes EcWRS-h14 and EcW-tRNA-TGADepositorInsertEcWRS-h14
ExpressionBacterialMutationS8A, V144G, V146CPromotertacIAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DYNLL1_Dynein-light
Plasmid#110006PurposeProtein expression and purification of DYNLL1_Dynein-lightDepositorInsertDYNLL1_Dynein-light (DYNLL1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only