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pET28a-SARS-CoV-2 NSP13

Plasmid

#176492

Purpose

Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

Depositor

Xiaodong Cheng

Article

White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)

Tags

His6 and T7

Expression

Bacterial

Mutation

I570 from SARS-CoV NSP13 was mutated to V to conv…

Promoter

T7

Available Since

Jan. 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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DHX9A

Plasmid

#42456

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DHX9A (DHX9 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pEK19

Plasmid

#210578

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdBIP1pro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-EGFR

Plasmid

#222610

Purpose

Expression of MAC3-tagged EGFR

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFR (EGFR Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pENO1-dTom-NATr

Plasmid

#52208

Purpose

Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

Depositor

Robert Wheeler

Article

Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

Insert

dTomato

Expression

Bacterial and Yeast

Mutation

codon optimized for Candida species

Promoter

ENO1

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pBAD-luxKate

Plasmid

#229946

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKate

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTU1-B-SP44s-GUS

Plasmid

#172430

Purpose

Expresses Streptomyces codon-optimised beta-D-glucuronidase

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

beta-D-glucuronidase

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

SP44s

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMH610

Plasmid

#131862

Purpose

Produces holo-PhyB* in E. coli

Depositor

Wilfried Weber

Article

Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

Inserts

PhyB(1-651)-RGD-His6-Cys
HO1
PcyA

Expression

Bacterial

Promoter

T7 promoter-1 and T7 promoter-2

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkGD

Plasmid

#13673

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

Gateway cassette

Use

Co-expression

Tags

GST

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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FepA

Plasmid

#26039

Depositor

Johann Deisenhofer

Article

Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

Insert

FepA (fepA E Coli)

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only