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pH6HTC_CCDC124-Halo

Plasmid

#175328

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC124 (CCDC124 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_2

Plasmid

#103943

Purpose

Protein expression and purification of PDZK1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_3

Plasmid

#103944

Purpose

Protein expression and purification of PDZK1 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his_flag

Plasmid

#210404

Purpose

For expression of VHH with C-terminal his and Flag tags

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

his6-flag

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-160

Plasmid

#118540

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET 2C-T10-dCasX

Plasmid

#126418

Purpose

E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

DpbCasX

Use

CRISPR

Tags

His-MBP

Expression

Bacterial

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-NLuc-MBP-6xHis

Plasmid

#135931

Purpose

Expresses NanoLuciferase in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSALECT-EcoBam

Plasmid

#59705

Purpose

Folding reporter with ampicillin resistance as a readout

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c2

Plasmid

#120873

Purpose

Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Promoter

lac

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTYB3-PUM1-HD

Plasmid

#17543

Depositor

Traci Hall

Article

Wang et al Mol Cell. 2001 Apr . 7(4):855-65.

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only