32,295 results
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Plasmid#196986PurposeFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpltetO1Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3
Plasmid#63199PurposeBioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET51b_HCAII
Plasmid#187110PurposeExpression of HCAII in bacteriaDepositorInsertHCAII
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAMK2D
Plasmid#38904PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.4t
Plasmid#210256PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCherry
superfolder GFP
TagsssraExpressionBacterialPromoteripaH7.8p and rpsMAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD-SYNZIP2
Plasmid#80658PurposeEncodes the synthetic coiled coil peptide SYNZIP2DepositorInsertSYNZIP2
Tags6xHisExpressionBacterialAvailable SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD-SYNZIP1
Plasmid#80647PurposeEncodes the synthetic coiled coil peptide SYNZIP1DepositorInsertSYNZIP1
Tags6xHisExpressionBacterialAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVSL10
Plasmid#201182PurposeBst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavableDepositorInsertBst polymerase large fragment (BF) F710Y/D598A
TagsHRV 3C protease cleavage site and His6ExpressionBacterialMutationF710Y D598APromoterT5-lacOAvailable SinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1320
Plasmid#84829PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only