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pKD227

Plasmid

#90954

Purpose

Expresses S. baltica TtrS under the Ptac promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Expression

Bacterial

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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tracrRNA(scr)-CJ8421_04975 mRNA

Plasmid

#199718

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and scrambled tracrRNA

Use

CRISPR

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pS23·Pm-GFP

Plasmid

#122593

Purpose

Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

Pm promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pm

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-PAmCherry-KanMX6

Plasmid

#87026

Purpose

Tag S. pombe genes in C terminal with PAmCherry

Depositor

Julien Berro

Insert

PAmCherry

Expression

Bacterial and Yeast

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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p403 – TDH3-MTS-mCherry-GFP1-10-His

Plasmid

#177730

Purpose

mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

mCherry-GFP1-10

Tags

Mitochondrial Targeting Sequence

Expression

Bacterial and Yeast

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGDP3 vatD

Plasmid

#112885

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

vatD

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTXB1-APEX2-F

Plasmid

#160083

Purpose

Bacterial expression of C-terminally FLAG-tagged APEX2 peroxidase

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

APEX2 peroxidase

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-AldoA

Plasmid

#224925

Purpose

Inducible expression of human aldolase A

Depositor

Liskin Swint-Kruse

Article

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

Aldolase A (ALDOA Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET16_Caspase-4_p10

Plasmid

#214308

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_p20_C258A

Plasmid

#214307

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

C258A

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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