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pCPP5741

Plasmid

#128722

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Insert

shcV-hopV1

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pOGG005

Plasmid

#113980

Purpose

Level 1 high copy number vector for E. coli expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET16b-Trx CC32/35SS

Plasmid

#36946

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin 1 (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

both active site cysteines (32,35) mutated to ser…

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AD

Plasmid

#108687

Purpose

Plasmid for assembly of donor DNA templates consisting of three expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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DDX20A

Plasmid

#42376

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DDX20A (DDX20 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pET51b(+)_LAMA-F98 (for GFP)

Plasmid

#130714

Purpose

Plasmid encoding the bacterial expression of LAMA-F98 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-F98

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTrc99A-Rat FABP1

Plasmid

#13577

Depositor

Martin Scanlon

Article

Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

Insert

Rat liver fatty acid binding protein (Fabp1 Rat)

Expression

Bacterial

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only
pQE-meGFP-DLC1

Plasmid

#192048

Purpose

Plasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFP

Depositor

Ionel Popa

Article

Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

Insert

DLC1 (DLC1 Human)

Expression

Bacterial

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-ATP

Plasmid

#225247

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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