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pNGFP-BC

Plasmid

#161956

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Tags

GFPuv His8

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCGFP-BC

Plasmid

#161959

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Tags

GFPuv His8

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBud-EGFP

Plasmid

#23027

Depositor

Andrea LeBlanc

Article

Jodoin et al PLoS One. 2009 . 4(8):e6647.

Insert

EGFP (eGFP )

Expression

Bacterial and Mammalian

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-NC

Plasmid

#30226

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with N and C terminus cleavages (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 399-478, 20-62

Available Since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only

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OPEN HLA-A*02:01-BirA

Plasmid

#215569

Purpose

E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN HLA-A*02:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

Glycine 120 to Cysteine

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-C5E-6XTUBE

Plasmid

#217628

Purpose

Bacterial expression of MBP tagged 6XTUBE

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

6XTUBE

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET_RGG

Plasmid

#124929

Purpose

Expression of RGG domain from LAF-1 gene

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

LAF-1 RGG domain (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

RGG domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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E2F-1 wt-pGex2TK

Plasmid

#21668

Depositor

William Kaelin

Article

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

E2F-1 (E2F1 Human)

Tags

GST

Expression

Bacterial

Mutation

wild type

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pBBR1k-GFPuv

Plasmid

#106382

Purpose

IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1

Plasmid

#173932

Purpose

Bacterial expression of ALC1

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1 (CHD1L Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Tags

7xHis

Expression

Bacterial

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAC572

Plasmid

#127973

Purpose

AMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

pyrG

Use

Fungal expression

Expression

Bacterial

Promoter

native promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pT7C-HSP90AB1

Plasmid

#177659

Purpose

Recombinant protein synthesis of human HSP90AB1

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSP90AB1 (HSP90AB1 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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p3034 GST-HPV-11 E6

Plasmid

#10848

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

HPV11 E6

Tags

GST

Expression

Bacterial

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pKL2299

Plasmid

#186332

Purpose

This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.

Depositor

Kan Wang

Article

Kang et al Front Plant Sci. 2022 May 4;13:860971. d

Insert

virulence genes from Agrobacterium tumefaciens pTiBo542

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAM5505

Plasmid

#132664

Purpose

pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Insert

MobA

Expression

Bacterial

Mutation

please see depositor comments below

Promoter

p1,p3

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_15bpLinker

Plasmid

#157786

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_15bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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