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pCOLA-T7-WspR:D70E

Plasmid

#79164

Purpose

Expresses constitutively active WspR D70E under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Mutation

D70E

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-p15a-pBAD-UTR1-TetR-T500

Plasmid

#45787

Depositor

Richard Murray

Insert

Tetracycline repressor

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pJJGL002

Plasmid

#180606

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

PlmCasX

Use

CRISPR

Tags

10xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAJT195

Plasmid

#202294

Purpose

Type IIS cloning plasmid for LPattB7 integrations w/ OriT

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

sfGFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pUdO4z

Plasmid

#210253

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO2z

Plasmid

#210251

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO1c

Plasmid

#210230

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Tags

His

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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KRigor980

Plasmid

#129763

Purpose

Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

Depositor

William Hancock

Insert

KHC (Khc Fly)

Tags

His6

Expression

Bacterial

Mutation

R210A

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pIKM1

Plasmid

#51054

Purpose

Kanamycin and erethromycin resistance

Depositor

Lee Lynd, Juergen Wiegel

Article

Tyurin et al Appl Environ Microbiol. 2004 Feb;70(2):8

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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