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pET11d_Rev

Plasmid

#119322

Purpose

Expression of protein Rev from HIV

Depositor

Francisco Blanco, Robert Tycko

Article

Blanco et al J Mol Biol. 2001 Nov 2;313(4):845-59. do

Insert

Rev (rev HIV B1)

Expression

Bacterial

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTXG

Plasmid

#52280

Purpose

Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Type

Empty backbone

Tags

GyrA-intein-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-cSrc-SH2-AviTag

Plasmid

#214230

Purpose

Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

Insert

cSrc kinase SH2 domain (SRC Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBP-P_cymR-PCymRC

Plasmid

#204071

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

cymR repressor and PCymRC cumic acid-inducible promoter

Expression

Bacterial

Mutation

None

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO PYL1 (36-211)

Plasmid

#177843

Purpose

Bacterial Expression of PYL1

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 1 (PYL1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_MANF-Halo

Plasmid

#175341

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

MANF (MANF Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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AlphaC

Plasmid

#124525

Purpose

pColE1_AlphaC

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET30b_T7Sav

Plasmid

#138588

Purpose

T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

Depositor

Markus Jeschek

Article

Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

Insert

T7-tagged streptavidin variant with native C-terminus

Use

Synthetic Biology

Tags

T7-tag

Expression

Bacterial

Promoter

PT7

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRSAN-QUE20C

Plasmid

#129316

Purpose

QUEEN ATP sensor for 20degC

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-20C

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Stat5B-SH2

Plasmid

#46516

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5B (STAT5B Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 593-692

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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