30,738 results

1. pET151-GFP-PP-RPN2 (940-953)

   Plasmid

   #73746

   Purpose

   Expression of GFP-PP-RPN2 (940-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 940-953 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pJMP8923

   Plasmid

   #220904

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pet22b-mEGFP

   Plasmid

   #166439

   Purpose

   Bacterial expression of 6x His tagged mEGFP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP
   

   Expression

   Bacterial

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pROP-PP-FBFP

   Plasmid

   #122137

   Purpose

   FMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)

   Depositor

   Srivatsan Raman

   Article

   Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

   Insert

   FBFP
   

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-FnCas12a-TEV

   Plasmid

   #174658

   Purpose

   FnCas12a expression plasmid

   Depositor

   Huimin Zhao, Jin Wang

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   FnCas12a
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Xla.Tubb2:hArr-TEV;he1.1:eYFP

   Plasmid

   #218859

   Purpose

   Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD131

   Plasmid

   #119886

   Purpose

   Optimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

   Insert

   pTet_mCherry
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6

   Plasmid

   #49795

   Purpose

   ePathBrick vector for pathway engineering in E. coli.

   Depositor

   Mattheos Koffas

   Article

   Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

   Type

   Empty backbone

   Tags

   S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBA620

   Plasmid

   #186240

   Purpose

   LbuCas13a Negative Control (RFP targeting)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   pTet-LbuCas13a + crRNA negative control
   

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBA630

    Plasmid

    #186241

    Purpose

    RfxCas13d Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-RfxCas13d + crRNA negative control
    

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. hUbc13

    Plasmid

    #51131

    Purpose

    human ubc13, E2 for synthesis k63 linked ubiquitin chain

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

    Insert

    ubc13 (UBE2N Human)
    

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pUC18-mini-Tn7T-Gm-lacZ

    Plasmid

    #63120

    Purpose

    mini-Tn7 lacZ transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. (SIM)10

    Plasmid

    #126946

    Purpose

    Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    

    Expression

    Bacterial

    Promoter

    unknown

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-mDyn3 pcDNA3

    Plasmid

    #36265

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 3 (Dnm3 Mouse)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

15. plRL58

    Plasmid

    #166886

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. plRL2

    Plasmid

    #163631

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET23a-H6-TEV-cAbl

    Plasmid

    #214234

    Purpose

    Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    ABL1 Kinase domain (ABL1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD24-fis

    Plasmid

    #51557

    Purpose

    E. coli fis ORF cloned into pBAD24 expression vector

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    FIS (fis E. coli)
    

    Expression

    Bacterial

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. cfSGFP2-anti-AlfaTag nanobody

    Plasmid

    #171818

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    cfSGFP2-anti-AlfaTag nanobody
    

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDule-tfmF A65V S158A

    Plasmid

    #85484

    Purpose

    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32V F108W Q109M I159M with 65Val and 158Ala

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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