30,040 results

1. pOTIII.EFGP.IGR3.6

   Plasmid

   #40866

   Depositor

   Harold Gainer

   Article

   Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

   Insert

   MOUSE OXYTOCIN GENE (Oxt Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   mouse oxytocin promoter

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKEW303-AcrVA5

   Plasmid

   #115657

   Purpose

   Expresses AcrVA5 in bacteria, under TetR control.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrVA5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKEW189-MBP-TEV-AcrVA1

   Plasmid

   #115660

   Purpose

   Expresses AcrVA1 in bacteria via T7 RNAP, for purification

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrVA1
   

   Use

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNAP

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyTag003-mKate2

   Plasmid

   #133452

   Purpose

   Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-mKate2
   

   Use

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRsetB-PercevalHR

   Plasmid

   #49081

   Purpose

   Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Tags

   7xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. AcrIIA2

   Plasmid

   #101042

   Purpose

   Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

   Depositor

   Zhiwei Huang

   Article

   Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

   Insert

   AcrIIA2
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK645

   Plasmid

   #71700

   Purpose

   Produces E. coli thioredoxin (EcTrxA)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGDP4 tet(A)

   Plasmid

   #112887

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   tet(A)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Sequence was codon optimized for E. coli K12 usin…

   Promoter

   Plac

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAWP78-PVCpnf_Δpvc10

   Plasmid

   #198448

   Purpose

   Produces PVCs containing truncated spike tip protein (Pvc10).

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_Δpvc10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-omega

    Plasmid

    #114632

    Purpose

    Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

    Depositor

    Anushree Chatterjee

    Article

    Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

    Insert

    dCas9-omega
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge