30,416 results
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Plasmid#124356PurposePlasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxinDepositorInsertgp41-1 N-intein and C-intein (bicistronic)
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationPromoterSTCAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNeae2
Plasmid#168300PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
UseTags6xHis, E-tag, and myc-tagExpressionBacterialMutationPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-J23111
Plasmid#113149PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A & H840APromoterAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minPro
Plasmid#211517PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minProAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGJJ045
Plasmid#183621PurposeabundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF1389
Plasmid#104962PurposeBacterial expression plasmid of bdSENP1 proteaseDepositorInsertSENP1
UseTagsHis14 tag and Tev protease cleavage siteExpressionBacterialMutationPromoterlacUV5Available SinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHN922
Plasmid#178975PurposeExpression of GST-His8-GFP-nanobodyDepositorInsertGST-His8-GFP-nanobody
UseAffinity Reagent/ AntibodyTagsGST, His8ExpressionBacterialMutationPromotertacAvailable SinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
UseTagsHisExpressionBacterialMutationPromoterAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP.R270
Plasmid#138219PurposeExpresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-DogCatcher
Plasmid#171929PurposeExpress Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.DepositorInsertCys-DogCatcher
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only