30,738 results

1. RpHLuorin2

   Plasmid

   #180227

   Purpose

   for bacterial expression of recombinant ratiometric pHluorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2
   

   Tags

   His-tag followed by thrombin cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET31b-T7-Spinach2

   Plasmid

   #79783

   Purpose

   Expresses Spinach2 under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pROP-PP-GFP

   Plasmid

   #122135

   Purpose

   GFP Reporter plasmid for B-Rex contain perfect operator sequence

   Depositor

   Srivatsan Raman

   Article

   Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

   Insert

   GFP
   

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFW2500

   Plasmid

   #224213

   Purpose

   Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Insert

   Fncpf1 and recT
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

   Plasmid

   #213527

   Purpose

   Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   D90A and E92A

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSF_his6_sumo_OC43_NSP14

   Plasmid

   #213519

   Purpose

   Expresses NSP14 of hCoV OC43

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV OC43 NSP14 (ORF1ab hCoV OC43)
   

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF_his6_sumo_NL63_NSP14

   Plasmid

   #213520

   Purpose

   Expresses NSP14 of hCoV NL63

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV NL63 NSP14 (ORF1ab hCoV NL63)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSF_his6_sumo_229E_NSP14

   Plasmid

   #213521

   Purpose

   Expresses NSP14 of hCoV 229E

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV 229E NSP14
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRAR074-control-sfGFP

   Plasmid

   #185032

   Purpose

   J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Expression

   Bacterial

   Mutation

   Nucleotides of rpmE 5'UTR scrambled, Only fi…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL103-SpCas9-FLAG-3XNLS

    Plasmid

    #182032

    Purpose

    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

    Depositor

    Steven Lin

    Article

    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

    Insert

    Streptococcus pyogenes cas9 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    12X His tag, 3XNLS, FLAG, and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTXB1-TnY

    Plasmid

    #163975

    Purpose

    IPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.

    Depositor

    Peter Smibert

    Article

    Liscovitch-Brauer et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

    Insert

    TnY
    

    Tags

    Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINB-AMSH*

    Plasmid

    #66712

    Purpose

    Expresses activated AMSH in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    hbp (Stam2 Mouse)
    AMSH (STAMBP Human)
    

    Tags

    His-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. 12.4kbVillin-ΔATG

    Plasmid

    #19358

    Depositor

    Deborah Gumucio

    Article

    Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

    Insert

    12.4kb Villin promoter
    

    Expression

    Bacterial

    Mutation

    No ATG - transgenes cloned downstream of the vill…

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBG100-TCPTP

    Plasmid

    #33365

    Depositor

    Ambra Pozzi

    Article

    Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

    Insert

    TCPTP-45 (PTPN2 Human)
    

    Tags

    6xHIS - cleavable

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

16. AARS_C2_pNIC-Bio3

    Plasmid

    #153047

    Purpose

    Expression construct for producing Avi tagged Alanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    AARS (AARS Human)
    

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN-sacB

    Plasmid

    #196249

    Purpose

    Plasmid containing the sacB gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    attR2-sacB-attL3
    

    Expression

    Bacterial

    Promoter

    sacB promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-mTurquoise2-CNA35

    Plasmid

    #61602

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mTurqouise and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSAM_Cam

    Plasmid

    #91570

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pL99

    Plasmid

    #78577

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Expression

    Bacterial

    Promoter

    inducible PnitA-NitR system

    Available Since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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