30,416 results

1. 1GFP/RNase H1 WT

   Plasmid

   #174447

   Purpose

   Express GFP-tagged RNase H1 WT in E. coli.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   RNase H1 (RNASEH1 Human)
   

   Use

   Tags

   His-tag, GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

   Plasmid

   #55215

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   2xStrep II and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-3c-rhVTN-N

   Plasmid

   #30225

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with N terminus cleavage (VTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids: 20*-62

   Promoter

   Available Since

   May 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST-KT

   Plasmid

   #44561

   Purpose

   for ectopic inducible expression of mycobacterial proteins

   Depositor

   Vinay Nandicoori

   Article

   Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

   Type

   Empty backbone

   Use

   E.coli-mycobacteria shuttle vector

   Tags

   Flag tag and His tag

   Expression

   Bacterial

   Mutation

   Promoter

   UV15 promoter

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. sfTq2ox-PBP5

   Plasmid

   #117960

   Purpose

   Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

   Depositor

   Tanneke den Blaauwen

   Article

   Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

   Insert

   sfTq2ox-PBP5 (dacA )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. plRL2

   Plasmid

   #163631

   Purpose

   Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Sac-Abeta(M1-42)

   Plasmid

   #71875

   Purpose

   Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-42) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRS-XylS-dCas9

   Plasmid

   #220196

   Purpose

   mini-Tn7 dCas9 expression vector - XylS/pM promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   XylS/pM

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET mCherry LIC cloning vector (u-mCherry)

   Plasmid

   #29769

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Use

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available Since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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