4,908 results

1. p4xLexAOp-O2

   Plasmid

   #165816

   Purpose

   Omega2 4xLexAOp for assembly of larger LexAOp response elements

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   4xLexAOp-O2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p4xLexAOp-O1

   Plasmid

   #165817

   Purpose

   Omega1 4xLexAOp for assembly of larger LexAOp response elements

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   4xLexAOp-O1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGal4BD 1-147

   Plasmid

   #165819

   Purpose

   1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Gal4BD 1-147
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGAL4AD 768-881

   Plasmid

   #165820

   Purpose

   768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   GAL4AD 768-881
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLexA BD

   Plasmid

   #165821

   Purpose

   LexA DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   LexA BD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQFBD

   Plasmid

   #165823

   Purpose

   QF DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   QFBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQFAD

   Plasmid

   #165824

   Purpose

   QF activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   QFAD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGGGSx4

   Plasmid

   #165807

   Purpose

   Flexible peptide linker of 4 repeats of GGGS, for assembly of TL1 to TL5 parts

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   GGGSx4
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT2A

   Plasmid

   #165809

   Purpose

   Viral T2A linker element for assembly of TL3 to TL5 parts

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   T2A
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGGSx4 NT

    Plasmid

    #165810

    Purpose

    Flexible peptide linker of 4 repeats of GGS, for assembly of N-terminal TL1 tags to TL3 parts

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    GGSx4 NT
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Promoterless TP10

    Plasmid

    #165787

    Purpose

    Drosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectors

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Promoterless T10
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pR20A02

    Plasmid

    #165791

    Purpose

    GB2.0 compatible FlyLight Enhancer element R20A02 in enhancer grammar

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    R20A02
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pR70B04

    Plasmid

    #165792

    Purpose

    GB2.0 compatible FlyLight Enhancer element R70B04 in enhancer grammar

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    R70B04
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pR76H03

    Plasmid

    #165793

    Purpose

    GB2.0 compatible FlyLight Enhancer element R76H03in enhancer grammar

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    R76H03
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMCS-TCCC

    Plasmid

    #165774

    Purpose

    Multiple cloning site part for enhancer cloning, requires minimal promoter element

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    MCS-TCCC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pdSCP

    Plasmid

    #165776

    Purpose

    Minimal synthetic Drosophila core promoter, requires enhancer element for full expression

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    dSCP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

    Plasmid

    #172914

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7c-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

    Plasmid

    #172913

    Purpose

    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Insert

    jGCaMP7b-T2A-tdTomato
    

    Use

    Tags

    6xHis and T2A-tdTomato

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAW-BiP-Nb127D01-mCherry-KDEL

    Plasmid

    #171575

    Purpose

    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
    

    Use

    Tags

    BiP signal peptide and mCherry-KDEL

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFASTbac-HisGST-PTPIP51(36-470)

    Plasmid

    #170531

    Purpose

    Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 )
    

    Use

    Tags

    GST tag and His tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge