5,046 results

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874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159671

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

ExuL-NLS-hSpCas9-T2A-GFP

Tags

T2A-GFP

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159672

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

βTub-NLS-hSpCas9-T2A-GFP

Tags

T2A-GFP

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::Vkg::mCherry

Plasmid

#163929

Purpose

Expression of GrabFP-ECM under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Mutation

G1767D

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_mCherry-CD8-vhhGFP

Plasmid

#163930

Purpose

Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_mCherry::Nrv1::vhhGFP4

Plasmid

#163926

Purpose

Expression of GrabFP-B-intracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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44 pLIB_12HIS

Plasmid

#160186

Purpose

N-term 3C cleavable 12xHis tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

12xHis

Expression

Insect

Promoter

polyhydrin

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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907L

Plasmid

#160237

Purpose

FlightN-ttgR-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

TtgR repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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46 pLIB_3C_C_HIS

Plasmid

#160188

Purpose

C-term 3C cleavable His tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

12xHis

Expression

Insect

Promoter

polyhydrin

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

Ubiquitin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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R979-X58-008: Hs.KRAS4b(1-188)

Plasmid

#159691

Purpose

Baculovirus protein expression of KRAS4b (1-188)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-188) (KRAS Human)

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Plasmid

#159692

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Depositor

Dominic Esposito

Article

Procter et al Protein Expr Purif. 2018 Nov;151:99-105.

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

M188L

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X05-636: His6-MBP-tev-G-Hs.KRAS4b(1-188)

Plasmid

#159553

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMT[Hygro]_GBP-3xFlag-DEST

Plasmid

#157813

Purpose

A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection

Depositor

Zoltan Lipinszki

Article

Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:

Insert

GFP-binding protein

Use

For drosophila cells

Tags

3xFlag

Expression

Insect

Promoter

metallothionein

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050E (Ubiq-CasRx)

Plasmid

#132416

Purpose

Express CasRx under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

CasRx

Expression

Insect

Promoter

Ubiq

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050G (white)

Plasmid

#132420

Purpose

express arrays of gRNA targeting White under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

white gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050J (GFP)

Plasmid

#133304

Purpose

express arrays of gRNA targeting GFP under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

GFP gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-CmTMC1truncated

Plasmid

#154908

Purpose

For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

Depositor

Motoyuki Hattori

Article

Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

Insert

GFP-CmTMC1truncated

Tags

8histidine-EGFP

Expression

Insect

Mutation

Truncations of the residues 1-243 and 843-969

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pVL1393-MAL-SYK

Plasmid

#157990

Purpose

Expresses SYK in insect cell (sf9)

Depositor

Byung Il Lee

Article

Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

Insert

Spleen tyrosine kinase (SYK Human)

Tags

Met-Ala-Leu and add Leu,Glu residues +His tag

Expression

Insect

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSBL_DK193

Plasmid

#156424

Purpose

Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein (Q57H)

Tags

sfGFP-His

Expression

Insect

Mutation

Q57H

Promoter

Polyhedrin

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-MuTMC2truncated

Plasmid

#154909

Purpose

For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

Depositor

Motoyuki Hattori

Article

Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

Insert

GFP-MuTMC2truncated

Tags

8histidine-EGFP

Expression

Insect

Mutation

Truncations of the residues 1-179 and 691-716

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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AlphaB

Plasmid

#124524

Purpose

pColE1_AlphaB

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlphaD

Plasmid

#124526

Purpose

pColE1_AlphaD

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBFv-nosP-Cas9

Plasmid

#138402

Purpose

Expresses Cas9 in the germline of Drosophila

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Insert

Cas9-NLS

Expression

Insect

Promoter

UAS

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1-LRRK2 WD40

Plasmid

#139211

Purpose

Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

Depositor

Hao Wu

Article

Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

Insert

LRRK2 (LRRK2 Human)

Tags

N-His and MBP

Expression

Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-Rab10-FLAG

Plasmid

#135559

Purpose

Express mouse Rab10 in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Rab10 (Rab10 Mouse)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM499

Plasmid

#134216

Purpose

Baculovirus expression of CENP-U CENP-Q CENP-OP

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPU + CENPQ+ CENPOP

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM498

Plasmid

#134215

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPT + CENPW

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM463

Plasmid

#134214

Purpose

Baculovirus expression of CENP-U and CENP-Q

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENP-U + Q

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM377

Plasmid

#134210

Purpose

Baculovirus expression of CENP-HKM and His-CENP-I

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-H/K/M untagged + his-I

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM610

Plasmid

#134218

Purpose

Baculovirus expression of CENP-R

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-R untagged

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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