4,958 results
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Plasmid#53473DepositorAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only
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pPTluc
Plasmid#87789PurposeLuciferase reporter for testing enhancer fragments in DrosophilaDepositorTypeEmpty backboneTagsluciferaseExpressionInsectPromoterminimal promoterAvailable SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT p85-GFP
Plasmid#126660PurposeInducible expression of p85-GFP in S2 cellsDepositorInsertPI3K SH2 containing subunit
ExpressionInsectAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM498
Plasmid#134215PurposeBaculovirus expression of CENP-T and CENP-WDepositorInserthis-CENPT + CENPW
ExpressionInsectAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B
Plasmid#176665PurposeExpression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC93
Plasmid#118911Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
TagseGFP-HIS6ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmFMR1_B
Plasmid#145940PurposeInsect Expression of DmFMR1DepositorInsertDmFMR1 (Fmr1 Fly)
ExpressionInsectMutationTwo amino acid mutations (N45D and G558R) and thr…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nup98-pDONR221
Plasmid#124241PurposeGateway entry clone of Drosophila Nup98-96 cDNA.DepositorInsertDrosophila Nup98-96 (Nup98-96 Fly)
ExpressionInsectAvailable SinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67B MBP-tagged human Norrin ( 31–133)
Plasmid#132625Purposeexpresses MBP-tagged human Norrin ( 31–133) in Hi5 insect cellDepositorInsertMBP-Norrin ( 31–133)
ExpressionInsectAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18b
Plasmid#135554PurposeExpress rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.DepositorInsertMunc18b (Stxbp2 Rat)
Tags6xHis tag and TEV siteExpressionInsectPromoterpolyhedrin promoterAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
ninaE-dsocs
Plasmid#26857DepositorInsertSlow Termination of Phototransduction (stops Fly)
TagsmycExpressionInsectMutationThis STOPS construct contains a mutated/truncated…Available SinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGMR-QF#7m1
Plasmid#46130DepositorInsertQF#7m1
ExpressionInsectAvailable SinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFTSG
Plasmid#162389PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cellDepositorInsertthermostable GFP (TGP)
TagsHisExpressionInsectAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pattB-nsyb-MKII::GAL4DBDo
Plasmid#64721Purposegenerating transgenic TRIC fliesDepositorInsertMKIIK11A::GAL4DBDo
ExpressionInsectAvailable SinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
10XUAS IVS Jaws mVenus_tr
Plasmid#111553PurposeOptogenetics in Drosophila: UAS-induced expression of Jaws mVenusDepositorInsertJaws_mVenus_tr
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKF297
Plasmid#74774Purposetemplate for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-ResistanceDepositorInsertTEV- 2xV5 tag (short version)
ExpressionInsectAvailable SinceApril 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::Nrv1::vhhGFP4
Plasmid#163926PurposeExpression of GrabFP-B-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP
Plasmid#32135DepositorInsertB2RT> STOP STOP B2RT>
ExpressionInsectAvailable SinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
907L
Plasmid#160237PurposeFlightN-ttgR-VP16-Sv40-attBDepositorInsertsTtgR repressor
Flightin promoter
TagsVP16ExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HFW-CRY2-VHH(GFP)
Plasmid#126027Purposeexpress Flag-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
TagsFlagExpressionInsectMutationWTPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM377
Plasmid#134210PurposeBaculovirus expression of CENP-HKM and His-CENP-IDepositorInsertCENP-H/K/M untagged + his-I
ExpressionInsectAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGal80,y+
Plasmid#26851DepositorAvailable SinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAct-dCBEevoAPOBEC1
Plasmid#195514PurposeUbiquitous expression of dCBEevoAPOBEC1 in DrosophilaDepositorInsertdCBE-evoAPOBEC1
UseCRISPRExpressionInsectPromoteract5CAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-scFv
Plasmid#78900PurposeExpresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cellsDepositorInsertscFV-VP64
UseCRISPRTagsGFPExpressionInsectPromoterpActin (Drosophila)Available SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcUW51-gE2
Plasmid#11621DepositorInsertHSV-1 glycoprotein E
TagsHisExpressionInsectMutationdeleted amino acids 391-550Available SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMT-GFP-p150Dynactin
Plasmid#24283DepositorAvailable SinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
13XLexAop2 IVS Jaws mCherry_tr
Plasmid#111551PurposeOptogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherryDepositorInsertJaws_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPRxRF3
Plasmid#138386PurposeTagRFP-T knock-in cassetteDepositorInsertTagRFP-T
ExpressionInsectAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac FPH
Plasmid#1925DepositorAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry-CD8-vhhGFP
Plasmid#163930PurposeExpression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only