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Plasmid#145924PurposeInsect Expression of DmPenguinDepositorInsertDmPenguin (peng Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmXRN1_A
Plasmid#145874PurposeInsect Expression of DmXRN1DepositorInsertDmXRN1 (pcm Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]
Plasmid#86455PurposeNuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(K14A)
Plasmid#86562PurposeExpresses human full-length Akt1(K14A) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationK14A mutation in the PH domainPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
OA-986B
Plasmid#124999PurposeExpresses dCas9-VPR driven by the Ubiquitin-63E promoterDepositorInsertdCas9-VPR
UsePiggybackTagsExpressionInsectMutationPromoterUbiquitinAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC92
Plasmid#118910Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
UseTagseGFP-HIS6ExpressionInsectMutationPromoterpolHAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aalb_726
Plasmid#176661PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST-FOXO 3A
Plasmid#52983Purposeinsect expression of FOXO triple mutantDepositorInsertFOXO (foxo Fly)
UseTags3x HAExpressionInsectMutation3A, triple mutant, T44A S190A S259APromoterHsp70Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-STIL
Plasmid#80265Purposebaculovirus creation plasmid for GST-STILDepositorInsertSTIL (STIL Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUb LwaCas13a + LwaCas13a Guide RNA
Plasmid#176307PurposeExpresses LwaCas13a and its associated guide RNA in Drosophila cellsDepositorInsertLwaCas13a
UseTagsHA tagExpressionInsectMutationE656G, I1060V, D1076G- please see depositor comme…PromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_763
Plasmid#176658PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
AByF
Plasmid#111084PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInsertsY homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3 g Tc' v]
Plasmid#86446PurposepBAc transposon with transformation marker for Tribolium transgenesis; Rescues vermilion white eyed mutant to black eyes; (transformation plasmid)DepositorInsert3xP3 g Tc' v
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 sec
Plasmid#172062PurposeUAS vector to express GFP1-10 in the ER lumenDepositorInsertGFP1-10 sec
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159677PurposePlasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.DepositorInsert[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159673PurposePlasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertRcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
UseTagsT2A-GFPExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP2 CC+CKK
Plasmid#59039Purposefor baculovirus expression of CAMSAP2 CC+CKK in Sf9 cellsDepositorInsertCAMSAP2 CC+CKK domains (CAMSAP2 Human)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab35 WT
Plasmid#37741DepositorInsertRab35 (Rab35 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAc-HT-Dmp53
Plasmid#85698Purposeexpression of Drosophila p53 in Drosophila cellsDepositorInsertDmp53 (p53 Fly)
UseTagsHisExpressionInsectMutationPromoterAc5Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
DPP7 (3JYH)
Plasmid#25354PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertdpp07.029.492 (DPP7 Human)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceAug. 19, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIB_GST-nsp13 (SARS-CoV-2)
Plasmid#169190PurposeBaculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cellsDepositorInsertGST-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsGSTExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAFM-NbrWT
Plasmid#40830PurposeExpresses Flag, Myc tagged Nbr in Drosophila cellsDepositorInsertNbr (Nbr Fly)
UseTags3xFLAG and 6xMycExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
No-repeats
Plasmid#122487PurposeA control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)DepositorInsertLacZ
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVP16 AD
Plasmid#165822PurposeVP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertVP16 AD
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-DREDD-V5-His
Plasmid#63749PurposeExpresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorInsertDeath related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
UseTagsV5-6XHisExpressionInsectMutationPromoterpMTAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab11 DN
Plasmid#44831DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationS25N (dominant negative)PromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
HeatShock-acNPV_Bxb1_SV40UTR
Plasmid#87641PurposeFor creating drosophila transgenics expressing HeatShock-acNPV_Bxb1_SV40UTRDepositorInsertBxb1
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-GFP-Msp300KASH
Plasmid#170806PurposeExpresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesisDepositorInsertGFP-Msp300KASH
UseTagsGFPExpressionInsectMutationPromoter5xUAS + hsp70 minimal promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-Patronin CC+CKK
Plasmid#59048Purposefor baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)DepositorInsertPatronin (Patronin Fly)
UseTagseGFPExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-Patronin CC 534–1457
Plasmid#59050Purposefor baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)DepositorInsertPatronin (Patronin Fly)
UseTagseGFPExpressionBacterial and InsectMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only