4,931 results

1. pFB-CG-LYCHOS WT-HA-GFP

   Plasmid

   #199687

   Purpose

   Expression of LYCHOS WT in insect cells

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Tags

   10xHis, EGFP, HA, HRV 3C, and His

   Expression

   Insect

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. IR76b-T2A-QF2

   Plasmid

   #162523

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir76b gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Ir76b-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir76b-right-HDR-arm
   

   Expression

   Insect

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GR3-T2A-QF2

   Plasmid

   #162521

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Gr3 gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Gr3-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr3-right-HDR-arm
   

   Expression

   Insect

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. IR8a-T2A-QF2

   Plasmid

   #162520

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir8a gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Ir8a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir8a-right-HDR-arm
   

   Expression

   Insect

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFB-CG-LYCHOS FP>IA-HA-GFP

   Plasmid

   #199688

   Purpose

   Expression of LYCHOS FP>IA mutant in insect cells

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Tags

   10xHis, 6xHis, EGFP, HA, and HRV 3C

   Expression

   Insect

   Mutation

   F43 and P44 mutated to I and A respectively

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. OA-1067L

   Plasmid

   #200253

   Purpose

   pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato

   Depositor

   Omar Akbari

   Article

   Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

   Insert

   6 gRNAs targeting Doublesex, Intersex, bTublin
   

   Expression

   Insect

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pALuWBB

   Plasmid

   #183202

   Purpose

   dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA

   Depositor

   Mitch McVey

   Insert

   w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
   

   Use

   CRISPR; Recombines at attp landing site in fly ge…

   Expression

   Insect

   Promoter

   U6-3

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

   Plasmid

   #198037

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   E696K

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac-GST-TEV-mCherry-TBK1

    Plasmid

    #198033

    Purpose

    Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST-TEV and mCherry

    Expression

    Insect

    Mutation

    E696K

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ptAD-seq-ubi63E-Gal4-DBD

    Plasmid

    #111930

    Purpose

    Vector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.

    Depositor

    Alexander Stark

    Article

    Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

    Insert

    tAD-seq Gal4-DBD
    

    Expression

    Insect

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL4.26_4xUAS_DSCP_GFP

    Plasmid

    #111931

    Purpose

    Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

    Depositor

    Alexander Stark

    Article

    Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

    Insert

    4xUAS GFP
    

    Expression

    Insect

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-lambdaN-HA-DmGIGYF_1-640_AH

    Plasmid

    #148933

    Purpose

    Insect Expression of DmGIGYF1_1-640

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1_1-640 (Gyf Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-DmGIGYF_331-374-GST-V5His6_AH

    Plasmid

    #148934

    Purpose

    Insect Expression of DmGIGYF_331-374

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_331-374 (Gyf Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH

    Plasmid

    #148936

    Purpose

    Insect Expression of DmGIGYF1_641-1574

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1_641-1574 (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmGIGYF-F361AF367A_AH

    Plasmid

    #148937

    Purpose

    Insect Expression of DmGIGYF-F361AF367A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-F361AF367A (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-DmGIGYF-W349A_AH

    Plasmid

    #148938

    Purpose

    Insect Expression of DmGIGYF-W349A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-W349A (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH

    Plasmid

    #148939

    Purpose

    Insect Expression of DmGIGYF-WFFtoAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-WFFtoAAA (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmGIGYF-Y58AY60AM65A_AH

    Plasmid

    #148940

    Purpose

    Insect Expression of DmGIGYF1-Y58AY60AM65A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1-Y58AY60AM65A (Gyf Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-lambdaN-HA-DmGIGYF-YYMYFWFtoAAAAAAA_AH

    Plasmid

    #148941

    Purpose

    Insect Expression of DmGIGYF1-YYMFWFtoAAAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1-YYMFWFtoAAAAAAA (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAc5.1B-EGFP-DmHPat-PPFPPFtoAAAAAA_AH

    Plasmid

    #148908

    Purpose

    Insect Expression of DmHPat-PPFPPFtoAAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmHPat-PPFPPFtoAAAAAA (Patr-1 Fly)
    

    Expression

    Insect

    Mutation

    three silent mutations compared to the sequence g…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH

    Plasmid

    #148909

    Purpose

    Insect Expression of Dm4EHP-W114A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    Dm4EHP-W114A (4EHP Fly)
    

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAc5.1B-lambdaN-HA-DmGIGYF-C-del331-370-YYMYFWFtoAAAAAAA_AH

    Plasmid

    #148910

    Purpose

    Insect Expression of DmGIGYF-del331-370-YYMFWFtoAAAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-del331-370-YYMFWFtoAAAAAA (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAc5.1B-lambdaN-HA-DmGIGYF-del331-370_AH

    Plasmid

    #148911

    Purpose

    Insect Expression of DmGIGYF-del331-370

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-del331-370 (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAc5.1B-lambdaN-HA-DmGIGYF-del331-370-YFWFtoAAAA_AH

    Plasmid

    #148912

    Purpose

    Insect Expression of DmGIGYF-del331-370-YFWFtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-del331-370-YFWFtoAAAA (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5.1B-lambdaN-HA-DmGIGYF-YFWFtoAAAA_AH

    Plasmid

    #148913

    Purpose

    Insect Expression of DmGIGYF-YFWFtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-YFWFtoAAAA (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1B-EGFP-Dm4EHP_AH

    Plasmid

    #148914

    Purpose

    Insect Expression of Dm4EHP

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    Dm4EHP (4EHP Fly)
    

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1B-EGFP-Dm4EHP-R93PE139L_AH

    Plasmid

    #148915

    Purpose

    Insect Expression of Dm4EHP-R93PE139L

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    Dm4EHP-R93PE139L (4EHP Fly)
    

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAc5.1B-EGFP-Dm4EHP-V75AL91A_AH

    Plasmid

    #148916

    Purpose

    Insect Expression of Dm4EHP-V75AL91A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    Dm4EHP-V75AL91A (4EHP Fly)
    

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc5.1B-EGFP-Dm4EHP-W85A_AH

    Plasmid

    #148917

    Purpose

    Insect Expression of Dm4EHP-W85A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    Dm4EHP-W85A (4EHP Fly)
    

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAc5.1B-EGFP-DmGIGYF-del331-370_AH

    Plasmid

    #148921

    Purpose

    Insect Expression of DmGIGYF-del331-370

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-del331-370 (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAc5.1B-EGFP-DmGIGYF-YFWFtoAAAA_AH

    Plasmid

    #148922

    Purpose

    Insect Expression of DmGIGYF-YFWFtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF-YFWFtoAAAA (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAc5.1B-EGFP-DmMe31B-C285AL289A_AH

    Plasmid

    #148923

    Purpose

    Insect Expression of DmMe31B-C285AL289A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmMe31B-C285AL289A (me31B Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc5.1B-EGFP-DmMe31B-CLLKtoAAAA_AH

    Plasmid

    #148924

    Purpose

    Insect Expression of DmMe31B-CLLKtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmMe31B-CLLKtoAAAA (me31B Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAc5.1B-EGFP-DmMe31B-L310AK314A_AH

    Plasmid

    #148925

    Purpose

    Insect Expression of DmMe31B-L310AK314A

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmMe31B-L310AK314A (me31B Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc5.1B-HA-DmGIGYF_1-640-dsRNAres_AH

    Plasmid

    #148926

    Purpose

    Insect Expression of DmGIGYF1_1-640-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1_1-640-dsRNAres (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAc5.1B-HA-DmGIGYF-del331-370-YFWFtoAAAA-dsRNAres_AH

    Plasmid

    #148927

    Purpose

    Insect Expression of DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAc5.1B-HA-DmGIGYF-dsRNAres_AH

    Plasmid

    #148928

    Purpose

    Insect Expression of DmGIGYF1-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1-dsRNAres (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1B-HA-DmGIGYF-Y58AY60AM65A-dsRNAres_AH

    Plasmid

    #148929

    Purpose

    Insect Expression of DmGIGYF1-Y58AY60AM65A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF1-Y58AY60AM65A-dsRNAres (Gyf Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1B-lambdaN-HA-Dm4EHP_AH

    Plasmid

    #148930

    Purpose

    Insect Expression of Dm4EHP

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    Dm4EHP (4EHP Fly)
    

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge