4,958 results
-
Plasmid#50556PurposeExpression of luciferase reporter mRNA in S2 cellDepositorInsertLuciferase-let7-Poly(A)
UseLuciferaseExpressionInsectPromoterActin5CAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC92
Plasmid#118910Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
TagseGFP-HIS6ExpressionInsectPromoterpolHAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC6
Plasmid#118864Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFPDepositorInsertNcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, EGFPExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aalb_726
Plasmid#176661PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB-GluCl_cryst
Plasmid#31488DepositorInsertC. elegans glutamate-gated chloride channel alpha, crystallized construct
Tags8x-HisExpressionInsectAvailable SinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUb LwaCas13a + LwaCas13a Guide RNA
Plasmid#176307PurposeExpresses LwaCas13a and its associated guide RNA in Drosophila cellsDepositorInsertLwaCas13a
TagsHA tagExpressionInsectMutationE656G, I1060V, D1076G- please see depositor comme…PromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_763
Plasmid#176658PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
AByF
Plasmid#111084PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInsertsY homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right
ExpressionInsectAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASp-FMW-attB-AthHen1
Plasmid#104957PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 driversDepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 sec
Plasmid#172062PurposeUAS vector to express GFP1-10 in the ER lumenDepositorInsertGFP1-10 sec
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159677PurposePlasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.DepositorInsert[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159673PurposePlasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertRcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
TagsT2A-GFPExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSGE_MC19_Trl-2xUAS_dCP
Plasmid#71170PurposeLuciferase reporterDepositorInsertMC19_Trl-2xUAS_dCP
UseLuciferaseTagsluciferaseExpressionInsectPromoterDSCP Drosophila synthetic core promoterAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-QF
Plasmid#26269DepositorAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLIB_GST-nsp13 (SARS-CoV-2)
Plasmid#169190PurposeBaculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cellsDepositorInsertGST-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
TagsGSTExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVP16 AD
Plasmid#165822PurposeVP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertVP16 AD
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Cdc42 WT
Plasmid#52248PurposeDrosophila expression of YFP-Cdc42DepositorAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab5 DN
Plasmid#37694DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS43N (dominant negative)PromoterGAL4Available SinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSK32
Plasmid#72854Purposetemplate for C-terminal TEV-site + V5-tag, Puromycin-ResistanceDepositorInsertTEV-V5 tag
ExpressionInsectAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSGE_764_4xUAS_DOS_hkCP
Plasmid#71172PurposeLuciferase reporterDepositorInsert764_4xUAS_DOS_hkCP
UseLuciferaseTagsluciferaseExpressionInsectPromoterDSCP Drosophila synthetic core promoterAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only