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80,732 results

Showing: 2321 - 2340 of 80732 results

  2. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107942-plasmid-map-sequence-id-259760

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  5. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Article
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    96921-plasmid-map-sequence-id-181715

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  8. mCherry-Rab7a-7

    Plasmid
    #55127
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab7a (RAB7A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    N117S in NM_004637.5
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55127-plasmid-map-sequence-id-311825

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  13. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  18. mCherry-Golgi-7

    Plasmid
    #55052
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55052-plasmid-map-sequence-id-352458

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Showing: 2321 - 2340 of 80732 results