77,982 results

1. THBS1-bio-His

   Plasmid

   #53417

   Purpose

   Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   THBS1 (THBS1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-BAZ1B

   Plasmid

   #65372

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BAZ1B (BAZ1B Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEmaxNG-_2star_IVT

   Plasmid

   #227678

   Purpose

   plasmid for SpCas9-NG-PEmax** mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   NG-PEmax_2_star
   

   Use

   CRISPR

   Tags

   no

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-RT001-LVB

   Plasmid

   #163381

   Purpose

   Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   LaG17-VEEGFRtm-BFP (LVB)
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 - hSRSF6

   Plasmid

   #120419

   Purpose

   overexpression of human SRSF6

   Depositor

   Gillian Bates, Andreas Neueder

   Article

   Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

   Insert

   SRSF6 (SRSF6 Human)
   

   Expression

   Mammalian

   Promoter

   CMV immediate/early

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-hHDAC3

   Plasmid

   #98591

   Purpose

   3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCAS9-HDAC3 (HDAC3 Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE-UBC-mCD47-T2A-GFP

   Plasmid

   #205449

   Purpose

   lentiviral plasmid for expression of mouse CD47

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCD47 (Cd47 Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1 N-term GFP tTA

   Plasmid

   #107551

   Purpose

   All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

   Depositor

   David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP (optional)

   Expression

   Mammalian

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191796

   Purpose

   vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13X
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   R84A, H89A, R739A, R740A, H745A, H746A, H747A

   Promoter

   CBh, SV40, hU6

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

    Plasmid

    #182306

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    NFIA (NFIA Human)
    SOX9 (SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    TRE3G-Bi-directional

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. c-Myc-5-HT2A

    Plasmid

    #67944

    Purpose

    N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

    Depositor

    Javier Gonzalez-Maeso

    Article

    Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Tags

    c-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAVS-WT

    Plasmid

    #52135

    Purpose

    Retroviral vector for expression of human MAVS in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

    Insert

    MAVS (MAVS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1-P-CAG-DEST

    Plasmid

    #80490

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-pmEKAR4

    Plasmid

    #174440

    Purpose

    Plasma membrane-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEKAR4
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG

    Plasmid

    #217719

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2522

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+U1snRNA

    Plasmid

    #194202

    Purpose

    Expresses U1 snRNA

    Depositor

    Matthias Leisegang, Ralf Brandes

    Insert

    U1 snRNA (RNU1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEFBOS_CreIRESpuro

    Plasmid

    #183812

    Purpose

    Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

    Depositor

    Ed Stanley

    Article

    Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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