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pLX-EF1a_Empty

Plasmid

#221480

Purpose

Empty vector control for pLX307 overexpresion experiments

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-VE-Cadherin-N-10

Plasmid

#54959

Purpose

Localization: Tight Junctions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

VE-Cadherin (CDH5 Human)

Tags

mApple

Expression

Mammalian

Mutation

I503T

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMV-BC0525-luc2

Plasmid

#214655

Purpose

barcoded assay, Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered NFAT element with barcode 0525

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCI NL4-3 Nef-HA-WT

Plasmid

#24162

Depositor

Warner Greene

Article

Geleziunas et al Nature. 2001 Apr 12. 410(6830):834-8.

Insert

Nef (nef HIV)

Tags

HA

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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HaloTag-BRD4 Fusion Vector

Plasmid

#237019

Purpose

Express HaloTag(R)-BRD4 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

BRD4 (BRD4 Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Integrin β5-GFP

Plasmid

#205090

Purpose

Express GFP-tagged human integrin β5 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 5 (ITGB5 Human)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD9-SBP-EGFP

Plasmid

#222328

Purpose

Synchronize the trafficking of CD9 from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD9 fused to SBP-EGFP (CD9 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 deltaN89 Beta-catenin

Plasmid

#19288

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

N-terminal truncation, deleted aa 1-89

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA GFP10-ZIP

Plasmid

#182241

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP

Tags

split-GFP10 tripartite

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pTwist-SFFV-NFE2L1-Puro

Plasmid

#181917

Purpose

Lentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

NFE2L1 (NFE2L1 Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

SFFV

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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CRISPRoff-mScarletI

Plasmid

#217783

Purpose

Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

Depositor

Adriano Aguzzi

Article

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Insert

CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)

Tags

2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

Expression

Mammalian

Promoter

CAG

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA ZIP-GFP11

Plasmid

#182242

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP11

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

ZIP-GFP11

Tags

split-GFP11 tripartite

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMH-MYC

Plasmid

#101765

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

Myc

Expression

Mammalian

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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ANGPT-bio-His

Plasmid

#53404

Purpose

Expresses full-length Angiopoietin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ANGPT (ANGPT1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RUNX2

Plasmid

#192926

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX2 (RUNX2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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DYRK1A/pTRE3G-FL-hXIST

Plasmid

#149608

Purpose

Express doxycycline-inducible full length of human XIST cDNA

Depositor

Jeanne Lawrence

Article

Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

Insert

X-inactive specific transcript (XIST Human)

Expression

Mammalian

Promoter

pTRE3G

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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