78,002 results

1. pLenti-EML4-ALK variant 3a

   Plasmid

   #183829

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-dCas9-p300-P2A-PuroR

   Plasmid

   #83889

   Purpose

   Lentiviral Sp dCas9-p300-P2A-PuroR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
   pac from Streptomyces alboniger
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FASTHDR-tRNApromo-sgRNA-espCas91_1

   Plasmid

   #167210

   Purpose

   Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-TFR-20

   Plasmid

   #55144

   Purpose

   Localization: Transferrin Receptor, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   TFR (TFRC Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. PIK3CA - Exon20 H1047R

   Plasmid

   #16639

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Science. 2004 Apr 23. 304(5670):554.

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H1047R

   Available Since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304_CMV::mCherry-LgBiT-FLAG-NES

   Plasmid

   #199715

   Purpose

   Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   mCherry-LgBiT-FLAG-NES
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti PGK FKBP12(F36V)-OGT (Neo)

   Plasmid

   #154294

   Purpose

   Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x HA and FKBP12(F36V)

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

   Plasmid

   #209078

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   3K mutant: E35K, E46K, E61K

   Promoter

   TRE4G

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. tetO-SOX9

    Plasmid

    #170703

    Purpose

    doxycycline-inducible overexpression of SOX9

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SOX9 (SOX9 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ORFtag_TetR-SD_Frame2

    Plasmid

    #220982

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ORFtag_TetR-SD_Frame3

    Plasmid

    #220983

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Myc-GFP

    Plasmid

    #83375

    Purpose

    Expression of Myc tagged GFP

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    Myc-GFP
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

    Plasmid

    #209294

    Purpose

    mitoBE expression vector, target mtRNR2 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    SOD2-3xHA-TALE(RNR2-Left)-MutH
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. phL1A-pcDNA3

    Plasmid

    #12307

    Depositor

    Vance Lemmon

    Article

    Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

    Insert

    L1CAM (L1CAM Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-GRAM1b

    Plasmid

    #211494

    Purpose

    Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

    Insert

    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-PP7-2xmCherry-CAAX

    Plasmid

    #74925

    Purpose

    Expresses PP7-2xmCherry-CAAX

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    PP7-2xmCherry-CAAX
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-HA_N-human Prkd2

    Plasmid

    #137850

    Purpose

    Express Prkd2 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

    Insert

    Protein kinase d2 (PRKD2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEHAC1-HA-BirAonly

    Plasmid

    #232584

    Purpose

    Express HA-BirA

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    BirA
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-HIF1alpha P402A/P564A-pBabe-puro

    Plasmid

    #19005

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 402 to Alanin…

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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