82,515 results
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Plasmid#60490PurposeContains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
UseLuciferaseTagsLuciferase reporterExpressionMammalianMutationFour point mutations (G->C or A->C) in the …PromoterPartial HMG-CoA synthase promoter (-324/-225) fus…Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRP_ASC-LmCerulean
Plasmid#41840DepositorInsertapoptosis-associated speck-like protein containing CARD (PYCARD Human)
UseRetroviralTagsmCeruleanExpressionMammalianMutationStop Codon removedPromoterCMVAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRetroX GFP T2A Cre
Plasmid#63704PurposeTetracycline or doxycycline inducible expression of GFP T2A Cre fusion in mammalian cellsDepositorInsertT2A Cre
UseRetroviralTagsEGFPExpressionMammalianPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmTurquoise2-T2A-Venus(L68V)
Plasmid#60494PurposeProduces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmTurquoise2-T2A-mVenus(L68V)
TagsT2A peptideExpressionMammalianPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-mNaChBac-T2A-tdTomato
Plasmid#60650PurposeExpresses mNaChBac-T2A-tdTomatoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmNaChBac-T2A-tdTomato
ExpressionMammalianAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG_mRuby2_smFP FLAG
Plasmid#59760Purposeencodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRuby2_smFP_FLAG
ExpressionMammalianPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-CIB1-mCerulean-MP
Plasmid#58366PurposeFor use in light-induced protein inactivation through the interaction with CRY2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsmCeruleanExpressionMammalianPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEmerald-Nesprin3-C-18
Plasmid#54203PurposeLocalization: Nuclear Membrane , Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNesprin3 (SYNE3 Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pBT378_pattB-pCA-GFP-pA-attB
Plasmid#52554Purposefor PhiC31 integrase-mediated cassette exchangeDepositorInsertsPhiC31o attB (full length)
GFP
ExpressionMammalianAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF-Bos-FLAG RIG-I MIII
Plasmid#52877PurposeExpresses RIG-I with a mutation to motif III in mammalian cellsDepositorAvailable sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZT-AAVS1-L1
Plasmid#52637PurposeTALEN-mediated gene editing at human PPP1R12C/AAVS1 locusDepositorInsertLeft AAVS1 TALEN
UseTALENExpressionMammalianPromoterCMVAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMVMagFRET-1
Plasmid#50742PurposeExpresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgene encoding for MagFRET-1
ExpressionMammalianAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVITRO1-102.1F10-IgG1/λ
Plasmid#50366PurposeExpresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
ExpressionMammalianPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CENP-B mCherry
Plasmid#45219DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCENP-B mCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mRIP3 GFP wt
Plasmid#41382DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRIP3 (Ripk3 Mouse)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-H-Ras_V12
Plasmid#39504DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHRAS V12 (HRAS Human)
ExpressionMammalianMutationG12VPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-RosaR4 KKR mutations
Plasmid#37199DepositorInsertFokI (fused to RosaR4 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs R4, and SV40 NLSExpressionMammalianMutationI538K, E490K, and H537RPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMP-Luc
Plasmid#36394DepositorInsertFirefly Luciferase
UseRNAi and RetroviralExpressionMammalianAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-MRLC T18A,S19A
Plasmid#35681DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMRLC1 (MYL9 Human)
TagsEGFPExpressionMammalianMutationT18A and S19APromoterCMVAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-HA-LATS2-KR
Plasmid#33100DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertlats2 (Lats2 Mouse)
TagsHAExpressionMammalianMutationK655R, Kinase DeadPromoterCMVAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by